Mild stripping
Stripping Buffer, 1 liter
 15 g glycine
 1 g SDS
 10 ml Tween 20
Adjust pH to 2.2
Bring volume up to 1 L with ultrapure water.

Membrane incubation
  Use a volume that will cover the membrane. Incubate at room temperature for 5-10 minutes.
  Discard buffer.
  5-10 minutes fresh stripping buffer.
  Discard buffer.
  10 minutes PBS
  10 minutes PBS
  5 minutes TBST
  5 minutes TBST

Harsh stripping
Prepare buffer and strip membranes under a fumehood.
Buffer, 0.1 litre
20 ml SDS 10%
12.5 ml Tris HCl pH 6.8 0.5 M
67.5 ml ultra pure water
0.8 ml ß-mercaptoethanol

Procedure
1. Warm the buffer to 50° C.
2. Add the buffer to a small plastic box which has a tight lid. Use a volume that will cover the membrane.
3. Add the membrane. Incubate at 50° for up to 45 minutes with some agitation.
4. Dispose of the solution as required for ß-mercaptoethanol based buffers.
5. Rinse the membrane under running water tap for 1-2 hours.
6. Traces of ß-mercaptoethanol will damage the antibodies. Wash extensively for 5 minutes in TBST.