Protein Production
293FT, 293E, CHO

Truly Functional Protein
95% Purity
1-10 mg in 2 weeks

GeneExpressoMax™
293Expresso™

Transfection Reagents
* 90% Efficiency
* 95% Viability
* No sera interference
* Simple protocol
* High-throughput
* Only $98/ml

Baculovirus
Functional Protein
95% Purity
Fast turnaround
1-10 mg from Sf9 cells

Adenovirus, AAV
& Lentivirus

ORF or shRNA
* High Titer
* Cre, FLP, ΦC31
* Protein Kinases
* Transcription Factors
* Luciferases, GFP, RFP
* Protein Production
* Stable Cell Line


Excellgen

welgeninc.com adenovirus, AAV and retrovirus products and services

Adenovirus Services

Construct an adenovirus vector to express a gene (target cDNA provided by customer)
Construct an adenovirus vector to express a gene (target cDNA not provided by customer and will be custom cloned)
Generation of  adenovirus (adenoviral construct provided)
Gene silencing services- Insert a validated RNAi sequence into pQuiet vectors
Gene silencing services- Insert a validated RNAi sequence into an adenoviral vector
RNAi selection service
Amplification and purification of adenovirus
Plaque purification
Plaque assay to titer adenovirus
PCR assay for replication-competent adenovirus (RCA)
Assay for replication-competent adenovirus (RCA) using A549 cells
TCID50 (Tissue Culture Infectious Dose 50)
Adenoviral DNA Isolation
MOI Assay
 
Lentivirus Services

Mini-scale Lentivirus Production
Standard Lentivirus Production
Large-scale Lentivirus Production
Custom Lentiviral Construction and Production
Complete RNAi Service using Lentiviral vector
Lentivirus Titering Assay

Retrovirus and avian associated virus (AAV) Services

Mini-scale retroviral vector production
Standard-scale retroviral vector production
Large-scale retroviral vector production
Custom retroviral vector construction and production
Complete RNAi service based on retroviral vectors
Retrovirus titering Assay

Vectors
P1000     pLEP vector
P1100     pLEP-CMV
P2110     pQuiet-U6
P2120     pQuiet-H1
P2210     pQuiet-2U6
P2220     pQuiet-2H1
P2300     pQuiet-3
P2400     pQuiet-4
K1000     pSpeAd-recombinant adenovirus kit
K1100     pSpeAd-CMV recombinant adenovirus kit
K2110     pQuiet -U6 recombinant adenoviral vector construction kit
K2120     pQuiet -H1 recombinant adenoviral vector construction kit
K2210     pQuiet -2U6 recombinant adenoviral vector construction kit
K2220     pQuiet -2H1 recombinant adenoviral vector construction kit
K2300     pQuiet 3 recombinant adenoviral vector construction kit
K2400     pQuiet 4 recombinant adenoviral vector construction kit
C1000     pLEP-CMV-¦Â-gal vector
C1100     Supera competent cells
P3400     pQuiet-4-neo
P3300     pQuiet-3-neo
P3210     pQuiet-2U6-neo
P3120     pQuiet-H1-neo

Pre-made Adenoviruses
PRODUCT     CAT.#     TAG     PROMOTER     Species
Ad-CMV-Null     V1000      x     CMV      x
Ad- ¦Â-gal/lacZ     V1010      x     CMV     Bacteria
Ad-GFP     V1020      x     CMV      x
Ad-Luciferase     V1030      x     CMV     Firefly
Ad-scramble-shRNA     V1040      x     U6      x
Ad-Scramble-shRNA with GFP     V1050     GFP      x      x
Ad- ¦Â-gal-shRNA     V1060      x     U6      x
Ad-GFP-shRNA     V1070      x     U6      x
Ad-Luciferase-shRNA     V1080      x     U6      x
Ad-U6 Null     V1090      x     U6      x

December 4, 2011 at 7:38 pm

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Recombinant Lentivirus & Adenovirus
High Yield and High Titer up to 1010 (lentivirus) and 1013 (adenovirus) for Guaranteed Expression of GOI. $3000, $2500
Transient Protein Expression in CHO and HEK293 Cells
Transient Expression, Truly Functional Protein, 95% purity, 1~20 mg, fast turnaround. $5500, $3950
Baculovirus Protein Expression
Fast turn around, >95% purity functional protein. No outsourcing to China or India. $5500, $3950