Thermus thermophilus inorganic pyrophosphatase (PPi), recombinant
Available from http://www.labsupplymall.com
Description:
Thermus thermophilus PPi – recombinant form of the enzyme purified from E. coli strain carrying PPi Thermus thermophilus (strain HB8) overproducing plasmid. This enzyme catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate:
P2O7-4 + H2O –> 2HPO4-2.
Inhibition of PCR reaction by pyrophophatase can be prevented by addition of PPi into reaction mixture.
Storage and dilution buffer:
50 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.1 mM EDTA, 2 mM DTT, 50 % glycerol.
Unit definition:
One unit of activity is the amount of enzyme that will generate 1 µmol of phosphate per minute from pyrophosphate under standard reaction conditions.
Reaction buffer 10x:
PCR buffer of choice or sequencing buffer.
Applications:
PCR, RT-PCR and sequencing.
Quality control:
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.
Concentration:
2-5 units/µl
Storage:
-20 °C
