SuperFolder YFP for in vitro Bimolecular Fluorescence Complementation Assay
GFP 1-10 OPT: Y145F N105K I128T S30R E111V Y39I A206V S205T K166T I167V I171V
Cycle 3: M153T F99S V163A
EGFP: F64L
Structure: Dimer
Can be split into 2 fragments: 1-154 & 155-238 for in vitro bimolecular fluorescence
complementation (BiFC) assay. A linker with 24 amino acids can be inserted into these two fragments for bacteria expression. Fusion constructs are partly (ca. 40%) soluble,
could be purified and concentrated up to 70 mg/ml (YN fusions) or 20 mg/ml (YC fusions). Fusion proteins are stable after 5 thawing/freezing cycles.
Protein Sequence:
1 vskgeelftg vvpilveldg dvnghkfsvr gegegdatig kltlkfictt gklpvpwptl
61 vttlxlqcfa rypdhmkqhd ffksampegy vqertisfkd dgkyktravv kfegdtlvnr
121 ielkgtdfke dgnilghkle ynfnshnvyi tatrgtaaar packipndlk qkvmnhdkqk
181 ngikanftvr hnvedgsvql adhyqqntpi gdgpvllpdn hylsyqtvls kdpnekrdhm
241 vllefvtaag itlgmdelyk