Protein Production
293FT, 293E, CHO

Truly Functional Protein
95% Purity
1-10 mg in 2 weeks

GeneExpressoMax™
293Expresso™

Transfection Reagents
* 90% Efficiency
* 95% Viability
* No sera interference
* Simple protocol
* High-throughput
* Only $98/ml

Baculovirus
Functional Protein
95% Purity
Fast turnaround
1-10 mg from Sf9 cells

Adenovirus, AAV
& Lentivirus

ORF or shRNA
* High Titer
* Cre, FLP, ΦC31
* Protein Kinases
* Transcription Factors
* Luciferases, GFP, RFP
* Protein Production
* Stable Cell Line


Excellgen

SuperFolder YFP for in vitro Bimolecular Fluorescence Complementation Assay

GFP 1-10 OPT: Y145F N105K I128T S30R E111V Y39I A206V S205T K166T I167V I171V
Cycle 3: M153T F99S V163A
EGFP: F64L

Structure: Dimer

Can be split into 2 fragments: 1-154 & 155-238 for in vitro bimolecular fluorescence
complementation (BiFC) assay. A linker with 24 amino acids can be inserted into these two fragments for bacteria expression.  Fusion constructs are partly (ca. 40%) soluble,
could be purified  and concentrated up to 70 mg/ml (YN fusions) or 20 mg/ml (YC fusions). Fusion proteins are stable after 5 thawing/freezing cycles.

Protein Sequence:

        1 vskgeelftg vvpilveldg dvnghkfsvr gegegdatig kltlkfictt gklpvpwptl
       61 vttlxlqcfa rypdhmkqhd ffksampegy vqertisfkd dgkyktravv kfegdtlvnr
      121 ielkgtdfke dgnilghkle ynfnshnvyi tatrgtaaar packipndlk qkvmnhdkqk
      181 ngikanftvr hnvedgsvql adhyqqntpi gdgpvllpdn hylsyqtvls kdpnekrdhm
      241 vllefvtaag itlgmdelyk

July 31, 2010 at 8:11 am

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Baculovirus Protein Expression
Fast turn around, >95% purity functional protein. No outsourcing to China or India. $5500, $3950
Transient Protein Expression in CHO and HEK293 Cells
Transient Expression, Truly Functional Protein, 95% purity, 1~20 mg, fast turnaround. $5500, $3950