SuperFolder GFP (sfGFP) has
1, Cycle3 mutations: F99S, M153T, and V163A;
2, Enhanced GFP mutations: F64L and S65T;
3, Six additional mutations: S30R, Y39N, N105T, Y145F, I171V, A206V;
4, Q80R mutation

Characteristics of SuperFolder GFP:

1, 100% fluorescence recovery after denaturing by urea
2, Very bright
3, Faster chromophore formation

Sequence:

atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggt
 M  R  K  G  E  E  L  F  T  G  V  V  P  I  L  V  E  L  D  G
gatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggt
 D  V  N  G  H  K  F  S  V  R  G  E  G  E  G  D  A  T  N  G
aaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctg
 K  L  T  L  K  F  I  C  T  T  G  K  L  P  V  P  W  P  T  L
gtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcag
 V  T  T  L  T  Y  G  V  Q  C  F  A  R  Y  P  D  H  M  K  Q
catgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttccttt
 H  D  F  F  K  S  A  M  P  E  G  Y  V  Q  E  R  T  I  S  F
aaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggta
 K  D  D  G  T  Y  K  T  R  A  E  V  K  F  E  G  D  T  L  V
aaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataag
 N  R  I  E  L  K  G  I  D  F  K  E  D  G  N  I  L  G  H  K
ctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggc
 L  E  Y  N  F  N  S  H  N  V  Y  I  T  A  D  K  Q  K  N  G
attaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgat
 I  K  A  N  F  K  I  R  H  N  V  E  D  G  S  V  Q  L  A  D
cactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactat
 H  Y  Q  Q  N  T  P  I  G  D  G  P  V  L  L  P  D  N  H  Y
ctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctg
 L  S  T  Q  S  V  L  S  K  D  P  N  E  K  R  D  H  M  V  L
ctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatga
 L  E  F  V  T  A  A  G  I  T  H  G  M  D  E  L  Y  K  -

Availability of SuperFolder GFP

1, Gene synthesis

2, From BioBrick