Sodium Borate, Lithium Borate and Lithium Acetate Buffers for Fast Agarose Gel Electrophoresis.
1, Sodium Borate Buffer – 10 mM sodium boric acid (1X working solution is either 5 mM disodium borate decahydrate or 10 mM NaOH adjusted to pH 8.5 with boric acid, e.g, 8g NaOH, 47g boric acid in 1 L water to make 20X buffer, pH ~ 8.0).
2, Lithium Acetate Buffer – 5 mM lithium acetate, for separating DNA fragments >3 kb. (restriction digestion).
3, Lithium Borate Buffer – 1 mM lithium boric acid (lithium hydroxide pH buffered to pH ~8.5 with boric acid). For small DNA fragments (PCR products). Lithium Borate Buffer has better resolution than Sodium Borate Buffer for small DNA fragments.
Run gels at 200~ 300 V.
