Sequences of some fluorescent proteins
mRuby
Monomer
Derived from eqFP611: F102I; replaced 222CDLPSKLGRL231 with 222AGLGGG227
Excitation Maximum : 558 nm
Emission Maximum: 605 nm
Stokes shift: 47 nm
half-maturation time: 2.8 h
Quantum yield: 0.35
Molar extinction coefficient: 112,000 M-1 cm-1
Photobleaching probability: 5.36X 10-6
ATGAACAGCCTGATCAAAGAAAACATGCGGATGAAGGTGGTGCTGGAAGGCAGCGTGAAC 60
GGCCACCAGTTCAAGTGCACCGGCGAGGGCGAGGGCAACCCCTACATGGGCACCCAGACC 120
ATGCGGATCAAAGTGATCGAGGGCGGACCTCTGCCCTTCGCCTTCGACATCCTGGCCACA 180
TCCTTCATGTACGGCAGCCGGACCTTCATCAAGTACCCCAAGGGCATCCCCGATTTCTTC 240
AAGCAGAGCTTCCCCGAGGGCTTCACCTGGGAGAGAGTGACCAGATACGAGGACGGCGGC 300
GTGATCACCGTGATGCAGGACACCAGCCTGGAAGATGGCTGCCTGGTGTACCATGCCCAG 360
GTCAGGGGCGTGAATTTTCCCAGCAACGGCGCCGTGATGCAGAAGAAAACCAAGGGCTGG 420
GAGCCCAACACCGAGATGATGTACCCCGCTGACGGCGGACTGAGAGGCTACACCCACATG 480
GCCCTGAAGGTGGACGGCGGAGGGCACCTGAGCTGCAGCTTCGTGACCACCTACCGATCC 540
AAGAAAACCGTGGGCAACATCAAGATGCCCGGCATCCACGCCGTGGACCACCGGCTGGAA 600
AGGCTGGAAGAGTCCGACAACGAGATGTTCGTGGTGCAGCGGGAGCACGCCGTGGCCAAG 660
TTCGCCGGCCTGGGCGGAGGGTAA
EBFP2
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGAC 60
GGCGACGTAAACGGCCACAAGTTCAGCGTGAGGGGCGAGGGCGAGGGCGATGCCACCAAC 120
GGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACC 180
CTCGTGACCACCCTGAGCCACGGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAG 240
CAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC 300
TTCAAGGACGACGGCACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTG 360
GTGAACCGCATCGAGCTGAAGGGCGTCGACTTCAAGGAGGACGGCAACATCCTGGGGCAC 420
AAGCTGGAGTACAACTTCAACAGCCACAACATCTATATCATGGCCGTCAAGCAGAAGAAC 480
GGCATCAAGGTGAACTTCAAGATCCGCCACAACGTGGAGGACGGCAGCGTGCAGCTCGCC 540
GACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAGCCAC 600
TACCTGAGCACCCAGTCCGTGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTC 660
CTGCTGGAGTTCCGCACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA 720
mEmerald
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGAC 60
GGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTAC 120
GGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACC 180
CTCGTGACCACCTTGACCTACGGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAG 240
CAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC 300
TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTG 360
GTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCAC 420
AAGCTGGAGTACAACTACAACAGCCACAAGGTCTATATCACCGCCGACAAGCAGAAGAAC 480
GGCATCAAGGTGAACTTCAAGACCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCC 540
GACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC 600
TACCTGAGCACCCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTC 660
CTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA 720
mEos2
ATGAGTGCGATTAAGCCAGACATGAAGATCAAACTCCGTATGGAAGGCAACGTAAACGGG 60
CACCACTTTGTGATCGACGGAGATGGTACAGGCAAGCCTTTTGAGGGAAAACAGAGTATG 120
GATCTTGAAGTCAAAGAGGGCGGACCTCTGCCTTTTGCCTTTGATATCCTGACCACTGCA 180
TTCCATTACGGCAACAGGGTATTCGCCAAATATCCAGACAACATACAAGACTATTTTAAG 240
CAGTCGTTTCCTAAGGGGTATTCGTGGGAACGAAGCTTGACTTTCGAAGACGGGGGCATT 300
TGCATTGCCAGAAACGACATAACAATGGAAGGGGACACTTTCTATAATAAAGTTCGATTT 360
TATGGTACCAACTTTCCCGCCAATGGTCCAGTTATGCAGAAGAAGACGCTGAAATGGGAG 420
CCCTCCACTGAGAAAATGTATGTGCGTGATGGAGTGCTGACGGGTGATATTCATATGGCT 480
TTGTTGCTTGAAGGAAATGCCCATTACCGATGTGACTTCAGAACTACTTACAAAGCTAAG 540
GAGAAGGGTGTCAAGTTACCAGGCTACCACTTTGTGGACCACTGCATTGAGATTTTAAGC 600
CATGACAAAGATTACAACAAGGTTAAGCTGTATGAGCATGCTGTTGCTCATTCTGGATTG 660
CCTGACAATGCCAGACGATAA
mApple
ATGGTGAGCAAGGGCGAGGAGAATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAG 60
GTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGC 120
CGCCCCTACGAGGCCTTTCAGACCGCTAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCC 180
TTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGTCTACATTAAGCAC 240
CCAGCCGACATCCCCGACTACTTCAAGCTGTCCTTCCCCGAGGGCTTCAGGTGGGAGCGC 300
GTGATGAACTTCGAGGACGGCGGCATTATTCACGTTAACCAGGACTCCTCCCTGCAGGAC 360
GGCGTGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTA 420
ATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCGAGGAGCGGATGTACCCCGAGGACGGC 480
GCCCTGAAGAGCGAGATCAAGAAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGCCGCC 540
GAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACATCGTC 600
GACATCAAGTTGGACATCGTGTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAA 660
CGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAA
mKate-K230N = mTagRFP + R70K – N146S – F177L – H200R – K230N
ATGGTGTCTAAGGGCGAAGAGCTGATTAAGGAGAACATGCACATGAAGCTGTACATGGAG 60
GGCACCGTGAACAACCACCACTTCAAGTGCACATCCGAGGGCGAAGGCAAGCCCTACGAG 120
GGCACCCAGACCATGAGAATCAAGGTGGTCGAGGGCGGCCCTCTCCCCTTCGCCTTCGAC 180
ATCCTGGCTACCAGCTTCATGTACGGCAGCAAAACCTTCATCAACCACACCCAGGGCATC 240
CCCGACTTCTTTAAGCAGTCCTTCCCTGAGGGCTTCACATGGGAGAGAGTCACCACATAC 300
GAAGACGGGGGCGTGCTGACCGCTACCCAGGACACCAGCCTCCAGGACGGCTGCCTCATC 360
TACAACGTCAAGATCAGAGGGGTGAACTTCCCATCCAACGGCCCTGTGATGCAGAAGAAA 420
ACACTCGGCTGGGAGGCCTCCACCGAGATGCTGTACCCCGCTGACGGCGGCCTGGAAGGC 480
AGAAGCGACATGGCCCTGAAGCTCGTGGGCGGGGGCCACCTGATCTGCAACTTGAAGACC 540
ACATACAGATCCAAGAAACCCGCTAAGAACCTCAAGATGCCCGGCGTCTACTATGTGGAC 600
AGAAGACTGGAAAGAATCAAGGAGGCCGACAAAGAGACCTACGTCGAGCAGCACGAGGTG 660
GCTGTGGCCAGATACTGCGACCTCCCTAGCAATCTGGGGCACAAACTTAATTGA
