Protein Production
293FT, 293E, CHO
Truly Functional Protein
95% Purity
1-10 mg in 2 weeks

GeneExpressoMax™
293Expresso™
Transfection Reagents
* 90% Efficiency
* 95% Viability
* No sera interference
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* High-throughput
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Baculovirus
Functional Protein
95% Purity
Fast turnaround
1-10 mg from Sf9 cells
Adenovirus, AAV
& Lentivirus
ORF or shRNA
* High Titer
* Cre, FLP, ΦC31
* Protein Kinases
* Transcription Factors
* Luciferases, GFP, RFP
* Protein Production
* Stable Cell Line

Excellgen

Properties of red fluorescent proteins

Protein State Exmax Emmax e/l.mol-1.cm-1 QY pKa Maturation 30oC /h Photostability/s Brightness










mBanana monomer 540 553 6000 0.7 6.7 1 1.4 4
mKO “ 548 559 51600 0.6 5 4.5 122 31
E2-Orange tetramer 540 561 36500 0.54 4.5 1.3 81 20
mOrange monomer 548 562 71000 0.69 6.5 2.5 9 49
mKOk “ 551 563 105000 0.61 4.2 1.8 7 64
mOrange2 “ 549 565 58000 0.6 6.5 4.5 228 35
tdRFP2 dimer 552 579 120000 0.68 4.8 7 7 82
dTomato “ 554 581 138000 0.69 4.7 1 64 95
DsRed tetramer 558 583 75000 0.79 4.7 10 326 59
TagRFP monomer 555 584 98000 0.41 <4.0 1.7 37 40
TagRFP-T “ 555 584 81000 0.41 4.6 1.7 337 33
mTangerine “ 568 585 38000 0.3 5.7 7 5.1 11
E2-Red/Green tetramer 560 585 53800 0.67 4.5 1.2 93 36
DsRed-Express “ 554 586 30100 0.42 7 0.7 71 13
DsRed2 “ 561 587 43800 0.55 7 6.5 7 24
DsRedT3 “ 560 587 49500 0.59 7 1.3 7 29
DsRed-Max “ 560 589 48000 0.41 7 1.2 9 20
DsRed-Express2 “ 554 591 35600 0.42 7 0.7 64 15
DsRed-Monomer monomer 557 592 27300 0.14 7 1.3 15 4
mApple “ 568 592 75000 0.49 6.5 0.5 4.8 37
mStrawberry “ 574 596 90000 0.29 <4.5 0.8 11 26
mRFPmars “ 585 602 32600 0.25 7 7 7 8
mRFPruby “ 585 603 32300 0.25 7 7 7 8
mRuby “ 558 605 112000 0.35 5 2.8 179 39
mRFP1 dimer 584 607 50000 0.25 4.5 <1 8.7 13
mCherry monomer 587 610 72000 0.22 <4.5 0.25 135 16
KillerRed “ 585 610 45000 0.25 7 7 7 11
JRed “ 584 610 44000 0.2 5 slow 7 9
mKeima “ 440 620 14400 0.24 6.5 7 7 3
mRaspberry “ 598 625 86000 0.15 7 0.9 7 13
mKate2 “ 588 633 62500 0.4 5.4 <0.33 118 25
tdKatushka2 dimer 588 633 132500 0.37 5.4 <0.33 144 50
Katushka “ 588 635 65000 0.34 5.5 7 7 22
mKate monomer 588 635 31500 0.28 6.2 1.25 71 9
t-HcRed dimer 590 637 130000 0.04 7 7 7 5
HcRed tetramer 592 645 70000 0.05 7 7 7 4
mPlum monomer 590 649 41000 0.1 <4.5 1.7 77 4
AQ143 tetramer 595 655 90000 0.04 7 7 7 4

August 27, 2010 at 5:32 pm

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Baculovirus Protein Expression
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