Monitor DNA Transfection Efficiency using GFP and Blue Light Transilluminator DarkReader
1, Transfect your cells with a vector containing GFP.
2, Put your plate or dish on top of a DarkReader.
3, Take a picture of cells using a simple gel-documentation system from labsupplymall.com—you do not need an expensive fluorescence microscope to check transfection efficiency.
Example: Transfect Hela cells using GeneExpresso and PolyExpress in vitro DNA transfection reagent:
Of course you will see more clearly under fluorescence microscope:
Transfection in the presence of serum and antibiotics
Transfection in the absence of serum and antibiotics
