Fluorescent Protein E2-Crimson
E2-Crimson
from DsRed-Express2, noncytotoxic in bacteria and mammalian cells
Mature faster than other red fp (0.4 hr)
Brightness: 0.79 (mkate2: 0.6)
fast maturation, high photostability, high solubility, and low cytotoxicity
well-suited for in vivo applications involving sensitive cells such as primary cells and stem cells
tetramer
Substitutions relative to DsRed-Express2
E32V, Q66F, V71A, V73I, K83L, L85Q, F118L, L150N, I161N,
K163M, V175C, Y193H, S197Y
GCCACCATGGATAGCACTGAGAACGTCATCAAGCCCTTCATGCGCTTCAAGGTGCACATG 60
GAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGTGGGCGAGGGCAAGCCCTAC 120
GAGGGCACCCAGACCGCCAAGCTGCAAGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGG 180
GACATCCTGTCCCCCCAGTTCTTCTACGGCTCCAAGGCGTACATCAAGCACCCCGCCGAC 240
ATCCCCGACTACCTCAAGCAGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAAC 300
TTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCACCCTC 360
ATCTACCACGTGAAGTTCATCGGCGTGAACTTCCCCTCCGACGGCCCCGTAATGCAGAAG 420
AAGACTCTGGGCTGGGAGCCCTCCACTGAGCGCAACTACCCCCGCGACGGCGTGCTGAAG 480
GGCGAGAACCACATGGCGCTGAAGCTGAAGGGCGGCGGCCACTACCTGTGTGAGTTCAAG 540
TCCATCTACATGGCCAAGAAGCCCGTGAAGCTGCCCGGCTACCACTACGTGGACTACAAG 600
CTCGACATCACCTCCCACAACGAGGACTACACCGTGGTGGAGCAGTACGAGCGCGCCGAG 660
GCCCGCCACCACCTGTTCCAGTAG
MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAKLQVTKGGPLPFAWDI 60
LSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGTLIY 120
HVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHMALKLKGGGHYLCEFKSI 180
YMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHHLFQ
