Protein Production
293FT, 293E, CHO
Truly Functional Protein
95% Purity
1-10 mg in 2 weeks

GeneExpressoMax™
293Expresso™
Transfection Reagents
* 90% Efficiency
* 95% Viability
* No sera interference
* Simple protocol
* High-throughput
* Only $98/ml

Baculovirus
Functional Protein
95% Purity
Fast turnaround
1-10 mg from Sf9 cells
Adenovirus, AAV
& Lentivirus
ORF or shRNA
* High Titer
* Cre, FLP, ΦC31
* Protein Kinases
* Transcription Factors
* Luciferases, GFP, RFP
* Protein Production
* Stable Cell Line

Excellgen

Comparison of transfection efficiencies: GeneExpresso, LipoExpress, Lipofectamine 2000, and Expressfect

Comparison of transfection efficiencies of a few in vitro DNA transfection reagents: GeneExpresso, LipoExpress, Lipofectamine 2000, and Expressfect

GeneExpresso and LipoExpress are from Lab Supply Mall
Lipofectamine 2000 is from Invitrogen.

Expressfect is from Denville Scientific, Inc. (http://www.denvillescientific.com), Expressfect is an OEM product (SoFast) from China (http://www.sunmabio.com, http://www.actgene.com/Convoy.htm).

Lipofectamine 2000 LipoExpress
GeneExpresso Expressfect (SoFast)

Efficiency comparison of GeneExpresso, LipoExpress, Lipofectamine 2000, and Expressfect on Hela cells. A nuclear targeted GFP DNA was transfected with GeneExpresso, LipoExpress, Lipofectamine 2000, and Expressfect respectively per manufacturer’s protocols. The efficiency was checked 48 hours post transfection.
 
FACS Analysis

Conclusion:
Both GeneExpresso and LipoExpress have higher transfection efficiency than Lipofectamine 2000. Expressfect (OEM of SoFast) should not be used as transfection reagent.

May 21, 2009 at 3:19 pm

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Transient Protein Expression in CHO and HEK293 Cells
Transient Expression, Truly Functional Protein, 95% purity, 1~20 mg, fast turnaround. $5500, $3950
Recombinant Lentivirus & Adenovirus
High Yield and High Titer up to 1010 (lentivirus) and 1013 (adenovirus) for Guaranteed Expression of GOI. $3000, $2500
Baculovirus Protein Expression
Fast turn around, >95% purity functional protein. No outsourcing to China or India. $5500, $3950

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