Mammalian Protein Expression
Truly Functional Protein
95% Purity
Fast turnaround
Transient Expression
1-10 mg from CHO or 293 Cells
 Start from $3950


Baculovirus Protein Expression
Functional Protein
95% Purity
Fast turnaround
1-10 mg from insect Sf9 cells
 Start from $3950


High Titer Lentivirus
ORF, shRNA & Reporters
>10^9 titer
* Custom shRNA & cDNA Constructs
* Scramble shRNA
* Cre & FLP Recombinases
* Luciferases, Fluorescent Proteins
* Lentiviral Packaging Service
* Stable Cell Lines
* Induced Pluripotent Stem Cells


High Titer Adenovirus & AAV
ORF, shRNA & Reporters
>10^10 titer
* Custom shRNA & cDNA Constructs
* Scramble shRNA
* Cre & FLP Recombinases
* Luciferases, Fluorescent Proteins
* Transient Protein Expression
* Stable Cell Lines
* Induced Pluripotent Stem Cells

Excellgen

Comparison of transfection efficiencies: GeneExpresso, LipoExpress, Lipofectamine 2000, and Expressfect

Comparison of transfection efficiencies of a few in vitro DNA transfection reagents: GeneExpresso, LipoExpress, Lipofectamine 2000, and Expressfect

GeneExpresso and LipoExpress are from Lab Supply Mall
Lipofectamine 2000 is from Invitrogen.

Expressfect is from Denville Scientific, Inc. (http://www.denvillescientific.com), Expressfect is an OEM product (SoFast) from China (http://www.sunmabio.com, http://www.actgene.com/Convoy.htm).

Lipofectamine 2000 LipoExpress
GeneExpresso Expressfect (SoFast)

Efficiency comparison of GeneExpresso, LipoExpress, Lipofectamine 2000, and Expressfect on Hela cells. A nuclear targeted GFP DNA was transfected with GeneExpresso, LipoExpress, Lipofectamine 2000, and Expressfect respectively per manufacturer’s protocols. The efficiency was checked 48 hours post transfection.
 
FACS Analysis

Conclusion:
Both GeneExpresso and LipoExpress have higher transfection efficiency than Lipofectamine 2000. Expressfect (OEM of SoFast) should not be used as transfection reagent.

May 21, 2009 at 3:19 pm

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Baculovirus Protein Expression
Fast turn around, >95% purity functional protein. No outsourcing to China or India. $5500, $3950
Transient Protein Expression in CHO and HEK293 Cells
Transient Expression, Truly Functional Protein, 95% purity, 1~20 mg, fast turnaround. $5500, $3950
Recombinant Lentivirus & Adenovirus
High Yield and High Titer virus for Guaranteed Expression of GOI. $3000, $2500

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