Protein Production
293FT, 293E, CHO

Truly Functional Protein
95% Purity
1-10 mg in 2 weeks

GeneExpressoMax™
293Expresso™

Transfection Reagents
* 90% Efficiency
* 95% Viability
* No sera interference
* Simple protocol
* High-throughput
* Only $98/ml

Baculovirus
Functional Protein
95% Purity
Fast turnaround
1-10 mg from Sf9 cells

Adenovirus, AAV
& Lentivirus

ORF or shRNA
* High Titer
* Cre, FLP, ΦC31
* Protein Kinases
* Transcription Factors
* Luciferases, GFP, RFP
* Protein Production
* Stable Cell Line


Excellgen

Comparison of Red & Far-Red Fluorescence Proteins



FP excitation/emission maxima (nm) extinction coefficient (M−1 cm−1) quantum yield relative brightness extinction coefficient at 633 nm (M−1 cm−1) relative brightness with 633 nm excitation maturation half-time (h) photobleaching half-time (s) pKa
DsRed1 558/583 51500 0.71 1 11 –;
mPlum 587/649 29300 0.1 0.08 2300 0.02 1.6 31 ± 2 5.5
mRaspberry 594/627 42000 0.14 0.16 3600 0.03 2.1 21 ± 2 5
mKate2 586/630 56400 0.39 0.6 2300 0.04 0.8 23 ± 2 6.5
Katushka 584/631 76300 0.32 0.67 4600 0.03 0.6 15 ± 1 7.5
RFP637 585/637 53800 0.22 0.32 4600 0.03 2.4 60 ± 4 4.5
RFP639 587/639 74700 0.21 0.43 6700 0.05 1.7 29 ± 3 4.5
E2-Crimson 611/646 126000 0.23 0.79 55400 1 0.4 26 ± 3 4.5

July 29, 2010 at 11:50 am

Leave a Comment

*
To prove you're a person (not a spam script), type the security word shown in the picture. Click on the picture to hear an audio file of the word.
Click to hear an audio file of the anti-spam word


Sponsored Links Excellgen http://www.labsupplymall.com

Transient Protein Expression in CHO and HEK293 Cells
Transient Expression, Truly Functional Protein, 95% purity, 1~20 mg, fast turnaround. $5500, $3950
Recombinant Lentivirus & Adenovirus
High Yield and High Titer up to 1010 (lentivirus) and 1013 (adenovirus) for Guaranteed Expression of GOI. $3000, $2500
Baculovirus Protein Expression
Fast turn around, >95% purity functional protein. No outsourcing to China or India. $5500, $3950