Comparison of In Vitro Tranfection Reagents: PolyExpress™ and Lipofectamine 2000
PolyExpress™ DNA In Vitro Tranfection Reagent is a biodegradable polymer based DNA transfection reagent. It is effective for transfecting of common (HEK293, COS-7, NIH-3T3, HeLa, CHO) and a broad ranges of hard-to-transfect mammalian cells. PolyExpress™ reagent is capable to immobilize DNA migration during electrophoresis at very low concentration and form polyplexes within a few minutes at room temperature. Due to its biodegrable feature, the cationic polymer is rapidly degraded shortly after entering cells by endocytosis (Figure 1), therefore, it has much less cytotoxicity than other lipisome-based transfection reagent. PolyExpress™ (1.0 ml) is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
Figure 1. A Cartoon Showing Biodegradation of PolyExpress DNA Transfection Reagent After Endocytosis of Transfection Complex
Features
Bio-degradable after endocytosis
- Exceptional high titers of virus production
- Efficient for very long DNAs (>89 kb)
- Efficient for both single DNA transfection and multi DNA co-transfection
- High levels of recombinant protein production
- Simple and robust transfection procedure
- Very affordable
Storage Condition: Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Broad Transfection Spectrum for Mammalian Cell Types
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Cell Lines
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Efficiency (% GFP)
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Cell Lines
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Efficiency (% GFP)
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| McArdle 7777 | 65 -70% | SAOS-2 | 58% |
| Hep3D | 67-76% | SN56 | 81% |
| SHEP | 68-71% | MC3T3-E1 | 80% |
| 3T3-442A | 35% | Primary melanocyte | 35% |
| COS-7 | 85-90% | K562 | 38% |
| CV-1 | 60% | L929 | 59% |
| D 407 | 70% | MCF-7 | 68% |
| DHD Pro.b | 70% | MDCK | 68% |
| 3LL | 80% | Neuro2A | 86% |
| B16-F10 | 85% | NIH 3T3 | 76% |
| BAEC | 51% | PC12 | 30% |
| BHK-21 | 80% | SH-SY5Y | 25% |
| Ca Ski | 88% | SiHa | 60% |
| CaCo2 | 60% | SKOV3 | 65% |
| CHO | 88% | Huh-7 | 70% |
| HCS-2/8 | 61% | IGROV1 | 35% |
| HEK-293 | 86% | DF-1, C hicken Embryonic Cell | 50% |
| HeLa | 88% | 6CSFMEo | 71% |
| HLMEC | 72% | WEHI 231 | 26% |
| H-MVEC | 59% | A549 | 75% |
| Huh-7D12 | 72% | LNCap | 75% |
| ATT20 | 46% | Prim. mouse keratinocyte | 29% |
| SK-N-SH | 29% | Prim. human skin fibroblast | 50% |
| C2C12 | 46% | Prim. human pre-adipocyte | 32% |
| HepG2 | 72% | Prim. mouse embry. fibroblast | 30% |
Examples Showing Transfection Efficiency of PolyExpress™ DNA In Vitro Transfection Reagent on Common Cell Lines
| PolyExpress™ | Lipofectamine Plus |
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Fig. 2, Transfection efficiency comparison of PolyExpress™ vs. lipofectamine Plus on Chinese Hamster Ovary (CHO) cells. HA tagged beta-tubulin cDNA was delivered into CHO cells with PolyExpress™ (left panel) and lipofectamine Plus (right panel) respectively. FITC conjugated antibody against HA tag was utilized to pick up HA-beta-tubulin (Green) while a DM1a antibody was used to detect endogenous alpha-tubulin followed by probing with rhodamine conjugated secondary antibody (Red). (Courtesy of Dr. Shang Yin, University of Texas at Houston Medical School).
| PolyExpress™ | Lipofectamine 2000 |
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Fig. 3. A comparison showing transfection efficiency of PolyExpress™ reagent vs. a leading product, Lipofectamine 2000 on HEK293FT cells. HEK-293FT cells were transfected with GFP vector (pEGFP-N3) by PolyExpress™ (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
| PolyExpress™ | Lipofectamine 2000 |
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Fig. 4. A comparison showing transfection efficiency of PolyExpress™ reagent vs. a leading product, Lipofectamine 2000 on HepG2 cells. HepG2 cells were transfected with GFP vector (pEGFP-N3) by PolyExpress™ (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection
| PolyExpress™ | Lipofectamine 2000 |
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Fig. 5. A comparison showing transfection efficiency of PolyExpress™ reagent vs. a leading product, Lipofectamine 2000 on MDCK cells. MDCK cells are notoriously hard to transfect. With proprietary “Shaved Cell Transfection” protocol, PolyExpress™ (left panel) gives up to 70% GFP positive cells vs. Lipofectamine 2000 (right panel) around 5% efficiency. MDCK cells were transfected with GFP vector (pEGFP-N3) by PolyExpress™ (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 36 hours post transfection.
| PolyExpress™ | Fugene HD |
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Fig. 6. A comparison showing transfection efficiency of PolyExpress™ reagent vs. a leading product, Fugene HD on LNCap cells. LNCap cells were transfected with GFP vector (pEGFP-N3) by PolyExpress™ (left panel) and Fugene HD (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection
| PolyExpress™ DIC | PolyExpress™ FITC |
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Fig. 7. Neuro2A cells transfected with pEGFP-C1 plasmid using PolyExpress™ In Vitro DNA Transfection Reagent. The Neuro2A cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left) and FITC imaging (right) 24 hours post-transfection
| PolyExpress™ | Lipofectamine 2000 |
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Fig. 8. Comparison of cytotoxicity of PolyExpress ™ DNA In Vitro Transfection Reagent with L2K™ on primary murine skin fibroblast. The primary murine fibroblast was incubated with the indicated transfection reagents/pEGFP-C1 (DNA) complexes above for 4 hours in serum-free DMEM High Glucose medium followed by replacement of complete serum-containing medium. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging 24 hours post transfection
