GeneExpresso™ 2000 DNA In Vitro Tranfection Reagent is formulated with proprietary cationic polymer-lipid conjugagte to ensure that the transfection protocol is identical to Lipofetamine™ 2000.   GeneExpresso™  2000 was shown to efficiently deliver genes to various established cell lines as well as primary cells including HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines (Sf9 and Sf21) and a variety of other eucaryotic cell lines with less toxicity. GeneExpresso™ 2000 reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Features:

* No need to have major SOP change if you are currently using Lipofectamine 2000.
* Cell-dependent 3~20 times higher efficiency than competitor’s transfection reagents
* Efficient for hard-to-transfect cells
* Efficient for very long DNAs (up to 2.0 mb)
* Hight transfection efficiency in the presence of serum and antibiotics for most of cell types
* Exceptional high levels of recombinant protein production

Comparisons of Transfection Efficiency of GeneExpresso™ 2000 In Vitro DNA Transfection Reagent with the Leading Brand Name Products

GeneExpresso™ 2000 is top choice for hard-to-transfect cells. Efficiency comparison of GeneExpresso™ 2000 In Vitro DNA Transfection Reagent (left panel) with Lipofectamine 2000 (L2K, right panel) on primary cultured rat aortic smooth muscle cells. A nuclear targeted GFP DNA was transfected with GeneExpresso™ 2000 and L2K respectively per manufacturer’s protocols. The efficiency was checked 48 hours post transfection.

Left Panel: Comparison of GeneExpresso™ 2000 In Vitro DNA Transfection Reagent with leading products including Lipofectamine 2000 (L2K) and Lipofectamine LTX (LTX) on lentivirus production on 293T cells.
Right panel: Comparison of GeneExpresso™ 2000 In Vitro DNA Transfection Reagent with tranfection reagents from other vendors inluding Lipofectamine 2000 (L2K) and FuGene 6 on Hela cells. HeLa cells were transfected with plasmid DNA encoding GFP. Transfections were performed according to manufacturers recommendations and analysed by flow cytometry.

Exceptional high titers of lentivirus generated from 293T cells. Three DNAs co-transfected with GeneExpresso™ 2000 In Vitro DNA Transfection Reagent (left panel) in comparison of Lipofectamine LTX (right panel). 1.0 and 10 microliters virus supernatant from 293T cells were used to counter-infected 293F cells which was then passed FACS. The numbers at the upper right corner indicate the percentage of transduced cells. The titers of lentivirus generated with GeneExpresso™ 2000 and Lipofectamine LTX were quantified to be 2.1×10⁶ and 9.3×10⁶ tu/ml respectively.