Protein Production
293FT, 293E, CHO

Truly Functional Protein
95% Purity
1-10 mg in 2 weeks

GeneExpressoMax™
293Expresso™

Transfection Reagents
* 90% Efficiency
* 95% Viability
* No sera interference
* Simple protocol
* High-throughput
* Only $98/ml

Baculovirus
Functional Protein
95% Purity
Fast turnaround
1-10 mg from Sf9 cells

Adenovirus, AAV
& Lentivirus

ORF or shRNA
* High Titer
* Cre, FLP, ΦC31
* Protein Kinases
* Transcription Factors
* Luciferases, GFP, RFP
* Protein Production
* Stable Cell Line


Excellgen

Commonly used bacterial E.coli growth media

Commonly used bacterial E.coli growth media
LB (Luria Bertani) Miller broth (1%NaCl): 1% peptone, 0.5% yeast extract, and 1% NaCl
LB (Luria Bertani) Lennox Broth (0.5% NaCl): 1% peptone, 0.5% yeast extract, and 0.5% NaCl
SOB medium (Super Optimal Broth): 2% peptone, 0.5% Yeast extract, 10mM NaCl, 2.5mM KCl, 10mM MgCl2 , 10mM MgSO4
SOC medium (Super Optimal broth with Catabolic repressor): SOB + 20mM glucose
2x YT broth (2x Yeast extract and Tryptone): 1.6% peptone, 1% yeast extract, and 0.5% NaCl
TB (Terrific Broth) medium: 1.2% peptone, 2.4% yeast extract, 72 mM K2HPO4, 17 mM KH2PO4 and 0.4% glycerol
SB (Super Broth) medium: 3.2% peptone, 2% yeast extract, and 0.5% NaCl

High density bacterial E.coli growth media
DNAGro medium: Support E.coli growth to OD600 = 30 – 50. Increase plasmid DNA yield 10 times over LB
ProGro medium: Support E.coli growth to OD600 = 30 – 50. Increase protein yield 10 times over LB
AutoX medium: Support E.coli growth to OD600 = 30 – 50. Increase protein yield 10 times over LB without adding IPTG
DetoX medium: Reduce protein toxicity and support E.coli growth to OD600 = 30
InduX medium: Support E.coli growth to OD600 = 30. Increase protein yield, solubility, stability and activity
SecPro medium: Increase secretary protein expression over 100 times than LB or TB

February 20, 2012 at 10:04 am

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