Commonly used bacterial E.coli growth media
Commonly used bacterial E.coli growth media
LB (Luria Bertani) Miller broth (1%NaCl): 1% peptone, 0.5% yeast extract, and 1% NaCl
LB (Luria Bertani) Lennox Broth (0.5% NaCl): 1% peptone, 0.5% yeast extract, and 0.5% NaCl
SOB medium (Super Optimal Broth): 2% peptone, 0.5% Yeast extract, 10mM NaCl, 2.5mM KCl, 10mM MgCl2 , 10mM MgSO4
SOC medium (Super Optimal broth with Catabolic repressor): SOB + 20mM glucose
2x YT broth (2x Yeast extract and Tryptone): 1.6% peptone, 1% yeast extract, and 0.5% NaCl
TB (Terrific Broth) medium: 1.2% peptone, 2.4% yeast extract, 72 mM K2HPO4, 17 mM KH2PO4 and 0.4% glycerol
SB (Super Broth) medium: 3.2% peptone, 2% yeast extract, and 0.5% NaCl
High density bacterial E.coli growth media
DNAGro medium: Support E.coli growth to OD600 = 30 – 50. Increase plasmid DNA yield 10 times over LB
ProGro medium: Support E.coli growth to OD600 = 30 – 50. Increase protein yield 10 times over LB
AutoX medium: Support E.coli growth to OD600 = 30 – 50. Increase protein yield 10 times over LB without adding IPTG
DetoX medium: Reduce protein toxicity and support E.coli growth to OD600 = 30
InduX medium: Support E.coli growth to OD600 = 30. Increase protein yield, solubility, stability and activity
SecPro medium: Increase secretary protein expression over 100 times than LB or TB