1. Set up the following reaction in a 0.5 ml eppendorf tube by mixing the following reagents
gently and then spin down briefly to collect the reagents at the bottom of the tube.
Linearized vector (100-200 ng/μl):       6 μl
Purified PCR products (100-200 ng/μl):   n μl
10X CloneEZ® Buffer:                     2 μl
CloneEZ® Enzyme                          2 μl
Deionized water                   up to 20 μl

In general, add more than 10 μl PCR DNA (n = 10) to the reaction can produce nearly 95%
positive clones. In addition, less amount of DNA is appropriate for short PCR DNA fragments.
For different sizes of PCR DNA, different amount of DNA is recommended below:
PCR DNA of 1 kb:  4 μl
PCR DNA of 2 kb:  6 μl
PCR DNA of 3 kb:  8 μl
PCR DNA of >3 kb: 10 μl

2. Incubate the reactions at 22°C for 30 minutes, and then transfer tubes to ice and
incubate on ice for five minutes.

3. Proceed with transformation. The reaction can also be stored at -20°C for later transformation.