1, Transfect your cells with a vector containing GFP.
2, Put your plate or dish on top of  a DarkReader.
3, Take a picture of cells using a simple gel-documentation system from labsupplymall.com—you do not need an expensive fluorescence microscope to check transfection efficiency.

Example: Transfect Hela cells using GeneExpresso and PolyExpress in vitro DNA transfection reagent:

Of course you will see more clearly under fluorescence microscope:

Transfection in the presence of serum and antibiotics

Transfection in the absence of serum and antibiotics

Laminar Flow Hoods

* provide product protection only and must not be used when working with any form of biohazard or chemical hazard
* any potentially infectious aerosol that is created will lead to exposure of the operator and the environment
* horizontal-flow clean-air bench used for cell cultures can expose the researcher to aerosols of allergenic or infectious materials.
* vertical-flow clean-air bench also blows air out into the room

Biological Safety Cabinets

* provide personnel and environmental protection and commonly product protection
* infectious agents must be used in a biological safety cabinet NOT a laminar flow hood

Class I Biological Safety Cabinet

* a ventilated cabinet which provides personnel and environmental protection only
* air flow is directed away from the researcher, but is not HEPA filtered, therefore there is no product protection
* similar to a fume hood with a HEPA filter on the exhaust system to protect against the release of biohazards
* inward air flow ranges from 75-125 linear feet per minute (lfpm)
* can be used with radioisotopes and some toxic chemicals

Class II Biological Safety Cabinet

* provides personnel, product and environmental protection
* there are supply air and exhaust air HEPA filters
* two general types: IIA cabinets have a minimum inward air flow of 75 lfpm and recirculates 70% of the air; IIB cabinets have a minimum inward air flow of 100 lfpm and exhaust either 70% (type B1) or 100% (type B2)
* most of the biological safety cabinets at UVic are Class II

Class III Biological Safety Cabinet

* these cabinets provide personnel, product and environmental protection
* they are hermetically sealed and all procedures are conducted through arm-length rubber gloves
* used in high level (Level 4) containment labs
* there are two HEPA filters on the exhaust system

Here is how I search NCBI PubMed using script.

1, Write a script in Perl or Java to search pubmed by date and keyword.

2, If there is “next” link on the result page, let script to click on “next” link until there is no more “next” link.

3, extract abstract link from search result pages

4, pause 15~ 25  seconds between each click.

5, click each abstract page, make sure efetch is used, e.g.,

http://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pubmed&id=19501051&retmode=xml

http://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pmc&id=2708341

6, parse abstract using an XML parser.