Available from http://www.labsupplymall.com

Description:
Thermus thermophilus PPi - recombinant form of the enzyme purified from E. coli strain carrying PPi Thermus thermophilus (strain HB8) overproducing plasmid. This enzyme catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate:
P₂O₇^-4 + H₂O –> 2HPO₄^-2.
Inhibition of PCR reaction by pyrophophatase can be prevented by addition of PPi into reaction mixture.

Storage and dilution buffer:
50 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.1 mM EDTA, 2 mM DTT, 50 % glycerol.

Unit definition:
One unit of activity is the amount of enzyme that will generate 1 µmol of phosphate per minute from pyrophosphate under standard reaction conditions.

Reaction buffer 10x:
PCR buffer of choice or sequencing buffer.

Applications:
PCR, RT-PCR and sequencing.

Quality control:
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.

Concentration:
2-5 units/µl

Storage:
-20 °C

Popularity: 1%

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