Protein Production
293FT, 293E, CHO
Truly Functional Protein
95% Purity
1-10 mg in 2 weeks

GeneExpressoMax™
293Expresso™
Transfection Reagents
* 90% Efficiency
* 95% Viability
* No sera interference
* Simple protocol
* High-throughput
* Only $98/ml

Baculovirus
Functional Protein
95% Purity
Fast turnaround
1-10 mg from Sf9 cells
Adenovirus, AAV
& Lentivirus
ORF or shRNA
* High Titer
* Cre, FLP, ΦC31
* Protein Kinases
* Transcription Factors
* Luciferases, GFP, RFP
* Protein Production
* Stable Cell Line

Excellgen

Automated search of NCBI Pubmed databases

Here is how I search NCBI PubMed using script.

1, Write a script in Perl or Java to search pubmed by date and keyword.

2, If there is “next” link on the result page, let script to click on “next” link until there is no more “next” link.

3, extract abstract link from search result pages

4, pause 15~ 25  seconds between each click.

5, click each abstract page, make sure efetch is used, e.g.,

http://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pubmed&id=19501051&retmode=xml

http://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pmc&id=2708341

6, parse abstract using an XML parser.

July 26, 2009 at 9:24 pm

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