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293FT, 293E, CHO
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95% Purity
1-10 mg in 2 weeks

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293Expresso™
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* 90% Efficiency
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Baculovirus
Functional Protein
95% Purity
Fast turnaround
1-10 mg from Sf9 cells
Adenovirus, AAV
& Lentivirus
ORF or shRNA
* High Titer
* Cre, FLP, ΦC31
* Protein Kinases
* Transcription Factors
* Luciferases, GFP, RFP
* Protein Production
* Stable Cell Line

Excellgen

Alignment of new red & far red fluorescent proteins

                        10        20        30        40        50         60
EGFP           MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTG-KLPVPWPTL
mPlum      MVSKGEENNMAIIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTARLKVTKGGPLPFAWDIL
mRaspberry MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDIL
mKate              MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDIL
mKate2             MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKAVEGGPLPFAFDIL
mNeptune       MVSKGEELIKEDMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTGRIKVVEGGPLPFAFDIL
TagRFP657          MSELITENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTQRIKVVEGGPLPFAFDIL
                           70         80        90       100       110      120
EGFP               VTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLV
mPlum              SPQIQYGSKAYVKHPADIP–DYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFI
mRaspberry         SPQCQYGSKGYVKHPADIP–DYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFI
mKate              ATSFMYGSKTFINHTQGIP–DFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLI
mKate2             ATSFMYGSKTFINHTQGIP–DFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLI
mNeptune           ATCFMYGSKTFINHTQGIP–DFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLI
TagRFP657          ATSFMYGSHTFINHTQGIP–DFWKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLI
                         130       140       150       160       170       180
EGFP              NRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLAD
mPlum             YKVKVRGTNFPSDGPVMQKKTMG-WEASTERMYPE–DGALKGEMKMRLRLKDGGHYDAE
mRaspberry        YKVKLRGTNFPSDGPVMQKKTMG-WEASTERMYPE–DGALKGEMKMRLKLKDGGHYDAE
mKate             YNVKIRGVNFPSNGPVMQKKTLG-WEASTEMLYPA–DGGLEGRSDMALKLVGGGHLICN
mKate2            YNVKIRGVNFPSNGPVMQKKTLG-WEASTETLYPA–DGGLEGRADMALKLVGGGHLICN
mNeptune          YNVKIRGVNFPSNGPVMQKKTLG-WEASTETLYPA–DGGLEGRCDMALKLVGGGHLICN
TagRFP657         YNVKIRGVNFPSNGPVMQKKTLG-WEAHTEMLYPA–DGGLEGRTALALKLVGGGHLICN
                         190         200       210       220       230
EGFP              HYQQNTPIGD-GPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
mPlum             VKTTYMAKKP—VQLPGAYKTDIKLDITSH-NEDYTIVEQYERAEGRHSTGGMDELYK
mRaspberry        VKTTYMAKKP—VQLPGAYKTDIKLDITSH-NEDYTIVEQYERAEGRHSTGGMDELYK
mKate             LKTTYRSKKPAKNLKMPGVYYVDRRLERIKE-ADKETYVEQHEVAVARYCDLPSKLGHK
mKate2            LKTTYRSKKPAKNLKMPGVYYVDRRLERIKE-ADKETYVEQHEVAVARYCDLPSKLGHKLN
mNeptune          LKTTYRSKKPAKNLKMPGVYFVDRRLERIKE-ADNETYVEQHEVAVARYCDLPSKLGHKLN
TagRFP657         FKTTYRSKKPAKNLKMPGVYYVDYRLERIKE-ADKETYVEQHEVAVARYCDLPSKLGHKLN

July 29, 2010 at 12:23 pm

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