Adenoviral Gene Delivery vs Lentiviral Gene Delivery 

sacB  (sucrase) gene from Bacillus subtilis can replace attB gene for negative selection against incorrect recombinants and the parental donor vector following LR recombination.

attR1 –sacB—attR2

at gaacatcaaa aagtttgcaa aacaagcaac agtattaacc tttactaccg 1560
 cactgctggc aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa aagccatata 1620
 aggaaacata cggcatttcc catattacac gccatgatat gctgcaaatc cctgaacagc 1680
 aaaaaaatga aaaatatcaa gttcctgagt tcgattcgtc cacaattaaa aatatctctt 1740
 ctgcaaaagg cctggacgtt tgggacagct ggccattaca aaacgctgac ggcactgtcg 1800
 caaactatca cggctaccac atcgtctttg cattagccgg agatcctaaa aatgcggatg 1860
 acacatcgat ttacatgttc tatcaaaaag tcggcgaaac ttctattgac agctggaaaa 1920
 acgctggccg cgtctttaaa gacagcgaca aattcgatgc aaatgattct atcctaaaag 1980
 accaaacaca agaatggtca ggttcagcca catttacatc tgacggaaaa atccgtttat 2040
 tctacactga tttctccggt aaacattacg gcaaacaaac actgacaact gcacaagtta 2100
 acgtatcagc atcagacagc tctttgaaca tcaacggtgt agaggattat aaatcaatct 2160
 ttgacggtga cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa ggcaactaca 2220
 gctcaggcga caaccatacg ctgagagatc ctcactacgt agaagataaa ggccacaaat 2280
 acttagtatt tgaagcaaac actggaactg aagatggcta ccaaggcgaa gaatctttat 2340
 ttaacaaagc atactatggc aaaagcacat cattcttccg tcaagaaagt caaaaacttc 2400
 tgcaaagcga taaaaaacgc acggctgagt tagcaaacgg cgctctcggt atgattgagc 2460
 taaacgatga ttacacactg aaaaaagtga tgaaaccgct gattgcatct aacacagtaa 2520
 cagatgaaat tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac ctgttcactg 2580
 actcccgcgg atcaaaaatg acgattgacg gcattacgtc taacgatatt tacatgcttg 2640
 gttatgtttc taattcttta actggcccat acaagccgct gaacaaaact ggccttgtgt 2700
 taaaaatgga tcttgatcct aacgatgtaa cctttactta ctcacacttc gctgtacctc 2760
 aagcgaaagg aaacaatgtc gtgattacaa gctatatgac aaacagagga ttctacgcag 2820
 acaaacaatc aacgtttgcg cctagcttcc tgctgaacat caaaggcaag aaaacatctg 2880
 ttgtcaaaga cagcatcctt gaacaaggac aattaacagt taacaaataa

Comparison of Viral Delivery Systems

1, PlasMapper http://wishart.biology.ualberta.ca/PlasMapper/

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