ORFeome data can be downloaded from ftp://www.orfeomecollaboration.org/orfeome/orfeome_data/cumulative/

ORFeome Clones can be purchased from
Dana-Farber/Harvard Cancer Center
ASU’s DNASU Plasmid Repository
Invitrogen (Ultimate™ ORF)
GeneCopoeia

Summary of Vectors and Flanking Sequences in ORFeome Clones (10/1/08)

    (Sequences read from 5' (from the promoter) to 3' as they would appear in the vector)

Advantages of Excellgen’s LvExpress Lentivirus Production and Purification services

• Lightning Fast Cloning: Transfer GOI from Gateway donor vector to lentivirus vector in just 1 hour using Gateway LR Clonase. The latest hORFeome (v7.1) collection has 18,414 human ORFs representing at least 15,415 human genes. This set of libary or indvidual clone can be purchased from Dana-Farber/Harvard Cancer Center  or ASU’s DNASU Plasmid Repository.
• Reliable:  Generate high titer lentivirus in only 5 days even if the gene is toxic.
• Scalable:  Lentivirus production can be scaled up to 1 or 10 L of cultured cells.
• Fast Turnaround: Maximum 3 weeks are required for cloning,  generation of high titer lentivirus and virus purification.
• Strong CMV5 Promoter: Exceptional high level protein
  expression using CMV5-intron promoter in most cell types including
  dividing and non-dividing or primary cells.
• High Titer: from 10⁶ to 10¹⁰ TU/ml
• High Yield: up to 10¹¹ TU
• High Purity: Purify virus by FPLC or ultracentrifugation.
• Flexiable: shRNA for gene knockdown; cDNA: high level protein expression using CMV5 promoter.
• Promoter of Your Choice: mCMV, hCMV, EF-1alpha, CMV5, CAG, etc.
• Reporters: EGFP, monomeric GFP, RFP, iREP, Luciferases.
• Adenovirus-Lentivirus Hybrid System: Produce lentivirus in vivo from high titer adenovirus. Long-term expression of multiple genes with only one adenovirus vector.

LvExpress^TM Lentivirus Services:

1, Clone gene of interest into a lentivirus vector.
2, Large scale lentivirus production in 293 cells.
3. Lentivirus purification by ultracentrifugation and FPLC.
4, Titer determination.

AdExpress^TM Adenovirus System has been recently
co-developed by Excellgen and Feldan (http://www.feldan.com/), this new
adenovirus system offers the following advantages

• Lightning Fast Cloning: Transfer GOI from Gateway donor vector to adenovirus shuttle vector in just 1 hour using Gateway LR Clonase. The latest hORFeome (v7.1) collection has  18,414 human ORFs representing at least 15,415 human genes. This set of libary or indvidual clone can be purchased from Dana-Farber/Harvard Cancer Center  or ASU’s DNASU Plasmid Repository.
• Reliable:  Generate high titer crude adenovirus stock in only 5 days even if the gene is toxic.
• Scalable:  Adenovirus production can be scaled up to 1 or 10 L of cultured cells.
• Fast Turnaround: Maximum 2 weeks are required for cloning
  and generation of high titer crude adenovirus stock. Another 2 weeks
  are needed for large scale amplification and purification.
• RCA-Free: Special cell lines can be used to produce high quality replication-competent-free adenovirus.
• Strong CMV5 Promoter: Exceptional high level protein
  expression using CMV5-intron promoter in most cell types including
  dividing and non-dividing or primary cells.
• High Titer: Up to 10¹³ particles/ml or 10¹² PFU/ml.
• High Purity: Adenovirus purification by CsCl ultracentrifugation and FPLC.
• Flexiable: shRNA for gene knockdown; high level protein expression using CMV5 promoter.
• Promoter of Your Choice: mCMV, hCMV, EF-1alpha, CMV5, CAG, etc.
• Reporters: EGFP, monomeric GFP, RFP, iREP, Luciferases.
• High Capacity Vector: Reduce inflammation and prolong
  high-level transgene expression; GOI and promoter combined can be larger
  than 8 kb; Multiple genes can be expressed together in one vector.

AdExpress^TM Adenovirus Services:

1, Clone gene of interest directly into an adenovirus vector.
2, Produce crude adenovirus stock.
3, Large scale amplification, adenovirus purification by CsCl banding (ultracentrifugation) and FPLC.
4, Titer determination.
5. RCA assay.
6, Protein production in suspension cultured cells.
7. Production of high capacity helper-dependent (gut-less) adenovirus.

1. Make a HD adenovirus-lentivirus hybrid vector with Gateway cassette

-ITR–CMV-RFP-CMV-X-VSVG–CMV-Gag-Pol-Rev –5'LTR-Psi-PRE–CMV5-intron–attR1–CmR–ccdB–attR2-IRES-GFP-Puro-WPRE-3'SINLTR–ITR–Amp-Orig–

2. Make adenovirus

3. Make lentivirus using 293Ad cell line

1. Make a HD adenovirus-AAV hybrid vector with Gateway cassette

–N-Rep (P6, p19)–LoxP–stop—LoxP–C-Rep (P40)–Capsid –ITR–CMV–attR1–CmR–ccdB–attR2–ITR–Amp-Orig–

2. Make adenovirus

3. Make AAV using 293Cre cell line