RunBlue SDS Running Buffer( cat #: NXB50500)
20x Buffer
* 0.8 M Tricine
* 1.2 M Tris
* 2% SDS
* 50 mM Sodium Bisulfite

Tricine (free base), 71.70 g

Tris (free base), 72.60 g

SDS, 10.00 g

Sodium Bisulfite, 2.5 g

Ultra pure water to, 500 ml (~ 385 g)

The pH should be between 8.2 and 8.3 @ 25°C.

For Non-reduced samples(especially antibodies), omit the Sodium Bisulfite

For best results use low conductance ingredients to formulate the buffers. Do NOT use acid or base to adjust pH of buffers