UUU F 17.6 UCU S 15.2 UAU Y 12.2 UGU C 10.6
UUC F 20.3 UCC S 17.7 UAC Y 15.3 UGC C 12.6
UUA L 7.7 UCA S 12.2 UAA * 1.0 UGA * 1.6
UUG L 12.9 UCG S 4.4 UAG * 0.8 UGG W 13.2
CUU L 13.2 CCU P 17.5 CAU H 10.9 CGU R 4.5
CUC L 19.6 CCC P 19.8 CAC H 15.1 CGC R 10.4
CUA L 7.2 CCA P 16.9 CAA Q 12.3 CGA R 6.2
CUG L 39.6 CCG P 6.9 CAG Q 34.2 CGG R 11.4
AUU I 16.0 ACU T 13.1 AAU N 17.0 AGU S 12.1
AUC I 20.8 ACC T 18.9 AAC N 19.1 AGC S 19.5
AUA I 7.5 ACA T 15.1 AAA K 24.4 AGA R 12.2
AUG M 22.0 ACG T 6.1 AAG K 31.9 AGG R 12.0
GUU V 11.0 GCU A 18.4 GAU D 21.8 GGU G 10.8
GUC V 14.5 GCC A 27.7 GAC D 25.1 GGC G 22.2
GUA V 7.1 GCA A 15.8 GAA E 29.0 GGA G 16.5
GUG V 28.1 GCG A 7.4 GAG E 39.6 GGG G 16.5
Better one:
http://www.kazusa.or.jp/java/codon_table_java
Permalink
April 30, 2010 at 2:17 pm
·
Open wp-config.php file in wordpress root installation directory.
Add the following line:
define(’WP_POST_REVISIONS’, false);
Then delete revision history using SQL
DELETE FROM wp_posts WHERE post_type =’revision’;
Permalink
April 30, 2010 at 9:58 am
·
NBT-BCIP Stock:
Dissolve 188 mg NBT (Nitro blue tetrazolium chloride) and 94 mg BCIP (5-Bromo-4-chloro-3-indolyl phosphate, toluidine salt) in 10 ml of 67% DMSO (v/v).
Aliquote 1ml and store at -20 oC. NBT and BCIP can be purchased from Sigma or Gold Biotech
AP Buffer:
100mM NaCl, 0.1M Tris, 5mM MgCl2, adjust pH to 9.5 with hydrochloric acid.
Tris-HCl (MW 157.60) ———————- 15.8 g
NaCl (MW 58.44) ————————— 5.8 g
MgCl2 (MW 203.31) ———————— 1.02 g
Distilled water —————————– 900 ml
After washing to remove unbound 2nd antibody (alkaline phosphatase), add 5 ml AP buffer, 25 to 50 ul of NBT-BCIP Stock. Let color development for 10 minutes. Stop reaction by adding water and wash membrane with water.
Permalink
April 30, 2010 at 8:39 am
·
Dissolve 100 mg Coomassie Brilliant Blue G-250 (100 mg) in 50 ml 95% ethanol, add 100 ml 85% (w/v) phosphoric acid. Dilute to 1 liter when the dye has completely dissolved, and filter through Whatman #1 paper. Final concentrations in the reagent: 0.01% (w/v) Coomassie Brilliant Blue G-250, 4.7% (w/v) ethanol, 8.5% (w/v) phosphoric acid.
Permalink
April 29, 2010 at 7:07 pm
·
Composition: 0.12% Coomassie Blue G-250 , 10% ammonium sulfate, 10% phosphoric acid, and 20% methanol
Sensitivity: 1 ng.
Steps to Prepare Colloidal Silver Blue Protein Stain:
1, Add 100 ml of phosphoric acid to 500 ml ddH2O.
2, Add 100 g ammonium sulfate (powder) while stirring.
3, When the ammonium sulfate has dissolved, add 1.2 g Coomassie Blue G-250 (powder)
4, Add water to 800 ml.
5, While stirring, add 200 ml methanol.
6, Store the stain solution in a brown bottle. It is stable at room temperature for ~ 6 months.
Permalink
April 29, 2010 at 6:48 pm
·
