Mammalian Protein Expression
Truly Functional Protein
95% Purity
Fast turnaround
Transient Expression
1-10 mg from CHO or 293 Cells
Start from $3950


Baculovirus Protein Expression
Functional Protein
95% Purity
Fast turnaround
1-10 mg from insect Sf9 cells
Start from $3950


High Titer Lentivirus
ORF, shRNA & Reporters
>10^9 titer
* Custom shRNA & cDNA Constructs
* Scramble shRNA
* Cre & FLP Recombinases
* Luciferases, Fluorescent Proteins
* Lentiviral Packaging Service
* Stable Cell Lines
* Induced Pluripotent Stem Cells


High Titer Adenovirus & AAV
ORF, shRNA & Reporters
>10^10 titer
* Custom shRNA & cDNA Constructs
* Scramble shRNA
* Cre & FLP Recombinases
* Luciferases, Fluorescent Proteins
* Transient Protein Expression
* Stable Cell Lines
* Induced Pluripotent Stem Cells

Excellgen

Archive for October, 2009

Monitor DNA Transfection Efficiency using GFP and Blue Light Transilluminator DarkReader

1, Transfect your cells with a vector containing GFP.
2, Put your plate or dish on top of  a DarkReader.
3, Take a picture of cells using a simple gel-documentation system from labsupplymall.com—you do not need an expensive fluorescence microscope to check transfection efficiency.

Example: Transfect Hela cells using GeneExpresso and PolyExpress in vitro DNA transfection reagent:



Of course you will see more clearly under fluorescence microscope:


Transfection in the presence of serum and antibiotics


Transfection in the absence of serum and antibiotics

Comments

October 31, 2009 at 10:06 pm ·