Protein Production
293FT, 293E, CHO

Truly Functional Protein
95% Purity
1-10 mg in 2 weeks

GeneExpressoMax™
293Expresso™

Transfection Reagents
* 90% Efficiency
* 95% Viability
* No sera interference
* Simple protocol
* High-throughput
* Only $98/ml

Baculovirus
Functional Protein
95% Purity
Fast turnaround
1-10 mg from Sf9 cells

Adenovirus, AAV
& Lentivirus

ORF or shRNA
* High Titer
* Cre, FLP, ΦC31
* Protein Kinases
* Transcription Factors
* Luciferases, GFP, RFP
* Protein Production
* Stable Cell Line


Excellgen

Archive for October, 2009

Monitor DNA Transfection Efficiency using GFP and Blue Light Transilluminator DarkReader

1, Transfect your cells with a vector containing GFP.
2, Put your plate or dish on top of  a DarkReader.
3, Take a picture of cells using a simple gel-documentation system from labsupplymall.com—you do not need an expensive fluorescence microscope to check transfection efficiency.

Example: Transfect Hela cells using GeneExpresso and PolyExpress in vitro DNA transfection reagent:



Of course you will see more clearly under fluorescence microscope:


Transfection in the presence of serum and antibiotics


Transfection in the absence of serum and antibiotics

Comments

October 31, 2009 at 10:06 pm ·