Protein Production
293FT, 293E, CHO

Truly Functional Protein
95% Purity
1-10 mg in 2 weeks

GeneExpressoMax™
293Expresso™

Transfection Reagents
* 90% Efficiency
* 95% Viability
* No sera interference
* Simple protocol
* High-throughput
* Only $98/ml

Baculovirus
Functional Protein
95% Purity
Fast turnaround
1-10 mg from Sf9 cells

Adenovirus, AAV
& Lentivirus

ORF or shRNA
* High Titer
* Cre, FLP, ΦC31
* Protein Kinases
* Transcription Factors
* Luciferases, GFP, RFP
* Protein Production
* Stable Cell Line


Excellgen

Archive for May, 2007

Interesting biotech companies

Fluorescent Dyes from Algae

Martek manufactures and sells algae-derived, fluorescent dyes for use in protein and nucleic acid detection. Conjugated to various antibodies and small molecules, these products are ideal for use in a variety of applications including drug screening assays, microscopy, flow cytometry, and protein and gene chip imaging.

http://fluorescent.martek.com/

MycoAlert® Mycoplasma Detection Assay

http://www.lonzabioscience.com

http://www.lonzabioscience.com/Lonza_Catnav.oid.887.prodoid.Mycoalert

BioMagicTM Cloning Products

Bioefficiency Solutions, Inc: http://www.bioefficiencysolutions.com/contactus.html

Hunan Weiheng Biotech Co., Ltd

Contact Person:Mr. James Ting
Job Title:Sales Manager
Address: 2 Tongyinli, Changsha, China, Changsha, Hunan, China (Mainland) Zip/Postal Code:410011
Telephone:86-731-5404838
Fax:86-731-5404837
Website: http://www.hnexport.com

Gel staining: http://www.biotium.com/

EZBiolab (http://www.ezbiolab.com)

  • Protein Expression and Purification Service
  • Custom Polyclonal Antibody Production Service
  • EZSpin Plasmid Mini Prep Kit
  • Custom Gene Synthesis, $1.20/bp
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May 31, 2007 at 3:52 pm ·

Doing business in Maryland: Web resources

Comments

May 31, 2007 at 5:51 am ·

Memory Stick Media Compatibility Information for Sony Digital Cameras

Memory Stick® Media Compatibility Information for Sony Digital Cameras

Important Information:
  • This “Compatibility Chart” only provides compatibility testing results for the Memory Stick media applications listed in each product’s instruction manual with Memory Stick media types listed below.
  • This chart does not guarantee support for all Memory Stick media types’ performance nor compatibility among Memory Stick media compliant products.
  • Memory Stick media transmission speed varies by, and depends on, host hardware devices’ features and circumstances of use.
  • Attach a dedicated adapter to use Memory Stick Duo™ media in a slot for standard size media.
  • The Memory Stick media capacity indicated on the packaging will differ from actual usable capacity. A portion of the memory is used for data management file functions. Refer to the back of the packaging for the minimum guaranteed capacity. Click here to view the Memory Stick media user capacity chart.
  • Models listed in this color are discontinued.

Remarks:

*1 = Data over 128MB cannot be recorded in a single session.
*2 = For Memory Stick Duo media ONLY. Standard Memory Stick media cannot be used.
*3 = Transmission speed is restricted by the design of host hardware devices, equal to that of the Memory Stick PRO media.
*4 = Please disregard the ‘Busy’ message which may appear on the LCD screen during MPEG movie recording in VX Fine mode. Recording and playback operations, as well as recorded data, will not be affected.
*5 = A dedicated adapter must be attached to use Memory Stick Duo™ or MagicGate Memory Stick Duo media.
*6 = The MSAC-MMD adapter must be attached to use Memory Stick Micro™ media in a slot for Duo size Memory Stick Pro media.
*7 = This product is made by Sony Computer Entertainment Inc.
*8 = To use a Memory Stick Duo™, use the ADMSCF1 Memory Stick adapter.
*9 = The movie recording on the camera will stop when the maximum file size of approximately 2GB is reached.
*10 = The MSAC-MMS adapter must be attached to use the Memory Stick Micro™ media in a slot for standard size Memory Stick Pro media. Compatibility is not guaranteed when using the MSAC-MMD adapter in combination with another adapter.
*11 = The file system incorporated in the Memory Stick limits the capacity of a file to be recorded or played back to less than 4GB.
*12 = To use a Memory Stick Micro™ in a slot for CF devices, use the ADMSCF Memory Stick adapter with the MSAC-MMD Memory Stick adapter.

Legend:

X = No compatibility
O = Only data that does not require copyright protection (MagicGate™ copyright protection technology) can be recorded/played back.
C = Data that requires copyright protection (MagicGate™ copyright protection technology) can be recorded/played back.
- = Not guaranteed

Model Types Memory Stick® Memory Stick PRO™ Memory Stick PRO™ High Speed
Memory Stick PRO™
PSP™ Memory Stick™ Memory Stick Micro™(M2)
Standard Size 32MB/64MB/
128MB/
128MBX2 *1
256MB/512MB/
1GB
256MB/512MB/
1GB/2GB
256MB/512MB/
1GB/2GB
4GB
Duo Size 32MB/64MB/
128MB
256MB/512MB 256MB/512MB/
1GB/2GB
4GB 8GB *11 256MB/512MB/
1GB/2GB
32MB *7 1GB *7 256MB/
512MB/
1GB
Models
Cyber-shot® Cameras
DSC-F505 O*5 X X X X X X O*5 X X
DSC-F505V O*5 X X X X X X O*5 X X
DSC-F55E O*5 X X X X X X O*5 X X
DSC-F55K O*5 X X X X X X O*5 X X
DSC-F55V O*5 X X X X X X O*5 X X
DSC-F77 O*5 O*5 O*5 X X O*3*5 X O*5 O*5 0*10
DSC-F707 O*5 X X X X X X O*5 X X
DSC-F717 O*5 O*5 O*5 X X O*3*5 X O*5 O*5 0*10
DSC-F828 O*5 O*5 O*5 O*5*9 X O*3*5 O*3*9 O*5 O*5 0*10
DSC-F88 O*5 O*5 O*5 O*5*9 X O*3*5 O*3*9 O*5 O*5 0*10
DSC-FX77 O*5 O*5 O*5 X X O*3*5 X O*5 O*5 0*10
DSC-G1 O*2 O*2 O*2 O*9 O*9 O*2*3 X O O 0*6
DSC-H1 O*5 O*5 O*5 O*5*9 X O*3*5 O*3*9 O*5 O*5 0*10
DSC-H2 O*2 O*2 O*2 O*9 X O*2 X O O 0*6
DSC-H5 O*2 O*2 O*2 O*9 X O*2 X O O 0*6
DSC-H7 O*2 O*2 O*2 O*9 O*9 O*2*3 X O O 0*6
DSC-H9 O*2 O*2 O*2 O*9 O*9 O*2*3 X O O 0*6
DSC-L1 O*2 O*2 O*2*4 O*4*9 X O*2*3*4 X O O*4 0*6
DSC-M1 O*2 O*2 O*2 O*9 X O*2*3 X O O 0*6
DSC-M2 O*2 O*2 O*2 O*9 X O*2*3 X O O 0*6
DSC-N1 O*2 O*2 O*2 O*9 X O*2 X O O 0*6
DSC-N2 O*2 O*2 O*2 O*9 X O*2*3 X O O 0*6
DSC-P1 O*5 X X X X X X O*5 X X
DSC-P10 O*5 O*5 O*5 X X O*3*5 X O*5 O*5 0*10
DSC-P100 O*5 O*5 O*5 O*5*9 X O*3*5 O*3*9 O*5 O*5 0*10
DSC-P12 O*5 O*5 O*5 X X O*3*5 X O*5 O*5 0*10
DSC-P120 O*5 O*5 O*5 O*5*9 X O*3*5 O*3*9 O*5 O*5 0*10
DSC-P150 O*5 O*5 O*5 O*5*9 X O*3*5 O*3*9 O*5 O*5 0*10
DSC-P2 O*5 X X X X X X O*5 X X
DSC-P20 O*5 X X X X X X O*5 X X
DSC-P200 O*5 O*5 O*5 O*5*9 X O*3*5 O*3*9 O*5 O*5 0*10
DSC-P3 O*5 X X X X X X O*5 X X
DSC-P30 O*5 X X X X X X O*5 X X
DSC-P31 O*5 X X X X X X O*5 X X
DSC-P32 O*5 O*5 O*5 X X O*3*5 X O*5 O*5 0*10
DSC-P41 O*5 O*5 O*4*5 O*4*5*9 X O*3*5 O*3*9 O*5 O*4*5 0*10
DSC-P43 O*5 O*5 O*4*5 O*4*5*9 X O*3*5 O*3*9 O*5 O*4*5 0*10
DSC-P5 O*5 X X X X X X O*5 X X
DSC-P50 O*5 X X X X X X O*5 X X
DSC-P51 O*5 X X X X X X O*5 X X
DSC-P52 O*5 O*5 O*5 X X O*3*5 X O*5 O*5 0*10
DSC-P7 O*5 X X X X X X O*5 X X
DSC-P71 O*5 X X X X X X O*5 X X
DSC-P72 O*5 O*5 O*5 X X O*3*5 X O*5 O*5 0*10
DSC-P73 O*5 O*5 O*4*5 O*4*5*9 X O*3*5 O*3*9 O*5 O*4*5 0*10
DSC-P8 O*5 O*5 O*5 X X O*3*5 X O*5 O*5 0*10
DSC-P9 O*5 X X X X X X O*5 X X
DSC-P92 O*5 O*5 O*5 X X O*3*5 X O*5 O*5 0*10
DSC-P93 O*5 O*5 O*5 O*5*9 X O*3*5 O*3*9 O*5 O*5 0*10
DSC-R1 O*5 O*5 O*5 O*5*9 X O*5 O*9 O*5 O*5 0*10
DSC-S30 O*5 X X X X X X O*5 X X
DSC-S40 O*5 O*5 O*4*5 O*4*5*9 X O*5 O*9 O*5 O*4*5 0*10
DSC-S45 O*2 O*2 O*2 X X O*2*3 X O O 0*6
DSC-S50 O*5 X X X X X X O*5 X X
DSC-S500 O*2 O*2 O*2 X X O*2*3 X O O 0*6
DSC-S60 O*5 O*5 O*4*5 O*4*5*9 X O*5 O*9 O*5 O*4*5 0*10
DSC-S600 O*2 O*2 O*2 O*9 X O*2 X O O 0*6
DSC-S650 O*2 O*2 O*2 O*9 X O*2*3 X O O 0*6
DSC-S70 O*5 X X X X X X O*5 X X
DSC-S700 O*2 O*2 O*2 O*9 X O*2*3 X O O 0*6
DSC-S75 O*5 X X X X X X O*5 X X
DSC-S80 O*5 O*5 O*4*5 O*4*5*9 X O*5 O*9 O*5 O*4*5 0*10
DSC-S85 O*5 X X X X X X O*5 X X
DSC-S90 O*5 O*5 O*4*5 O*4*5*9 X O*5 O*9 O*5 O*4*5 0*10
DSC-ST80 O*5 O*5 O*4*5 O*4*5*9 X O*5 O*9 O*5 O*4*5 0*10
DSC-T1 O*2 O*2 O*2 O*9 X O*2*3 X O O 0*6
DSC-T10 O*2 O*2 O*2 O*9 X O*2*3 X O O 0*6
DSC-T100 O*2 O*2 O*2 O*9 O*9 O*2*3 X O O 0*6
DSC-T20 O*2 O*2 O*2 O*9 O*9 O*2*3 X O O 0*6
DSC-T3 O*2 O*2 O*2 O*9 X O*2 X O O 0*6
DSC-T30 O*2 O*2 O*2 O*9 X O*2 X O O 0*6
DSC-T33 O*2 O*2 O*2 O*9 X O*2*3 X O O 0*6
DSC-T5 O*2 O*2 O*2 O*9 X O*2 X O O 0*6
DSC-T50 O*2 O*2 O*2 O*9 X O*2*3 X O O 0*6
DSC-T7 O*2 O*2 O*2 O*9 X O*2 X O O 0*6
DSC-T9 O*2 O*2 O*2 O*9 X O*2 X O O 0*6
DSC-V1 O*5 O*5 O*5 X X O*3*5 X O*5 O*5 0*10
DSC-V3 O*5 O*5 O*5 O*5*9 X O*3*5 O*3*9 O*5 O*5 0*10
DSC-W1 O*5 O*5 O*5 O*5*9 X O*3*5 O*3*9 O*5 O*5 0*10
DSC-W100 O*2 O*2 O*2 O*9 X O*2 X O O 0*6
DSC-W12 O*5 O*5 O*5 O*5*9 X O*3*5 O*3*9 O*5 O*5 0*10
DSC-W15 O O O O*5*9 X O O*9 0*10
DSC-W17 O O O O*5*9 X O O*9 0*10
DSC-W200 O*2 O*2 O*2 O*9 O*9 O*2*3 X O O 0*6
DSC-W30 O*2 O*2 O*2 O*9 X O*2 X O O 0*6
DSC-W35 O*2 O*2 O*2 O*9 O*9 O*2*3 X O O 0*6
DSC-W40 O*2 O*2 O*2 O*9 X O*2 X O O 0*6
DSC-W5 O*5 O*5 O*5 O*5*9 X O*5 O*9 O*5 O*5 0*10
DSC-W50 O*2 O*2 O*2 O*9 X O*2 X O O 0*6
DSC-W55 O*2 O*2 O*2 O*9 O*9 O*2*3 X O O 0*6
DSC-W7 O*5 O*5 O*5 O*5*9 X O*5 O*9 O*5 O*5 0*10
DSC-W70 O*2 O*2 O*2 O*9 X O*2 X O O 0*6
DSC-W80 O*2 O*2 O*2 O*9 O*9 O*2*3 X O O 0*6
DSC-W90 O*2 O*2 O*2 O*9 O*9 O*2*3 X O O 0*6
Cyber-shot® U Cameras
DSC-U10 O*5 X X X X X X O*5 X X
DSC-U20 O*5 X X X X X X O*5 X X
DSC-U30 O*5 O*5 O*5 X X O*3*5 X O*5 O*5 0*10
DSC-U40 O*2 O*2 O*2 X X O*2*3 X O O 0*6
DSC-U50 O*2 O*2 O*2 X X O*2*3 X O O 0*6
DSC-U60 O*5 O*5 O*5 X X O*3*5 X O*5 O*5 0*10
Mavica® Cameras
MVC-FD92 O*5 X X X X X X O*5 X X
MVC-FD100 O*5 X X X X X X O*5 X X
MVC-FD200 O*5 X X X X X X O*5 X X
DSLR Cameras
DSLR-A100 O*2*4 O*2*4 O*2*4 O*4 O*4 O*2*4*8 X O*4 O*4 O*12

Ref: http://esupport.sony.com/EN/info/mspro-camera.html

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May 30, 2007 at 8:15 am ·

Use Oracle Physical Standby Database for backup

If your primary database is huge in size and has to be up all the time (24X7), but you want to make a cold backup. No problem, you can set up a physical standby database on another machine (site), and then use RMAN or traditional cold back up method to do a cold backup. The following are steps for doing a cold backup of standby database then restoring the backup to another host:

1, Shutdown standby database (server A):

sqlplus /nolog

connect sys/change_on_install@crm as sysdba
ALTER DATABASE RECOVER MANAGED STANDBY DATABASE CANCEL;
shutdown immediate;
exit;

2, Do a cold backup: copy data files, control files, init file, and redo log files to another server (server B).

3, Start standby database on server A:

sqlplus /nolog

connect sys/change_on_install@crm as sysdba
startup nomount;
alter database mount standby database;
recover managed standby database disconnect from session;
exit;

4, Create service, password file, spfile file for restoring cold backup files on server B:

oradim -DELETE -SRVC OracleServiceCRM

oradim -new -SRVC OracleServiceCRM -startmode M

del C:\oracle\ora92\database\PWDCRM.ora
orapwd file=C:\oracle\ora92\database\PWDCRM.ora password=change_on_install entries=30

sqlplus /nolog

connect sys/change_on_install@crm as sysdba
create spfile='C:\oracle\ora92\database\SPFILECRM.ORA' from pfile='C:\oracle\job\crmstandby\initCRM.ora';

5, Start Oracle database on server B

rem start service (windows) or process (*nix).

connect sys/change_on_install@crm as sysdba
STARTUP NOMOUNT;
ALTER DATABASE MOUNT STANDBY DATABASE;
recover managed standby database disconnect from session;

Use the following command if archive logs were copied manually from primary site to standby site for point-in-time recovery:

RECOVER STANDBY DATABASE;

Then convert standby database to primary database:

ALTER DATABASE RECOVER MANAGED STANDBY DATABASE CANCEL;
ALTER DATABASE RECOVER MANAGED STANDBY DATABASE FINISH;
ALTER DATABASE ACTIVATE STANDBY DATABASE;
SHUTDOWN IMMEDIATE;
STARTUP;
exit;

6, Make sure archive log destination is correct.

7, Create temporary data files for each temporary tablespace, use “reuse” option:

ALTER TABLESPACE temporary ADD DATAFILE 'K\oradata\crm\temp02.dbf' SIZE 2000M reuse;

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May 25, 2007 at 11:27 am ·

Restore RMAN cold backup to different node

PURPOSE

The purpose of this document is to demonstrate a restore of an RMAN cold backup to a different host than the original target.

SCOPE & APPLICATION

This note is intended for DBAs and Support Personnel.

NOTE: This document is using a MS Windows server, for a Liunx server, certain commands might have a different syntax.

Restoring an RMAN Backup to Another Node (server)
————————————————-

In certain circumstances, it may be desirable to restore a database from an RMAN backup onto a machine other than the original host.
* to recover data at a given point in time
* to duplicate a production instance
* to verify RMAN backup

Example
——–
Restore RMAN backup from Costpoint production server on us01ap18 to standby database server on us01ap19

The example assumes:

- the target database is on us01ap18
- the database is to be restored onto tus01ap19
- the RMAN catalog is installed on us01ap19.
- the directory structure of us01ap18 is the same as us01ap19
- the ORACLE_SID will not change for the restored database
- a recovery catalog is being used
- the backups were carried out to disk (for illustrative purposes, and to disassociate from any media manager specific issues)

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May 12, 2007 at 3:12 pm ·

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