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Protein Production
293FT, 293E, CHO
Truly Functional Protein
95% Purity
1-10 mg in 2 weeks

GeneExpressoMax™
293Expresso™
Transfection Reagents
* 90% Efficiency
* 95% Viability
* No sera interference
* Simple protocol
* High-throughput
* Only $98/ml

Baculovirus
Functional Protein
95% Purity
Fast turnaround
1-10 mg from Sf9 cells

Adenovirus, AAV
& Lentivirus
ORF or shRNA
* High Titer
* Cre, FLP, ΦC31
* Protein Kinases
* Transcription Factors
* Luciferases, GFP, RFP
* Protein Production
* Stable Cell Line

Excellgen

Induction of iPSCs from human fibroblasts using adenovirus

Reprogramming of Mouse Embryonic Fibroblasts with Stem Cell Factors in Lentivirus

Stem cell factors pricing

Use iPS cells to model diseases

Reprogramming somatic cells to iPS cells

Reprogramming Somatic Cells to Induced Pluripotent Stem Cells

Protein Expression using L-arabinose as inducer

EF1 alpha promoter sequence

Protein Expression in bacteria (vectors and host strains)

Excellgen’s Custom Adenovirus Services

Qbiogene Q-mate™ Inducible Expression System

AAV vectors from Stanford University Deisseroth Lab

Adeno-Associated Virus (AAV) from UNC Vector Core as AAV1, AAV2, AAV2.5, AAV5, AAV6, AAV8 & AAV9

Memorial Sloan-Kettering Cancer Center Gene Transfer & Somatic Cell Engineering

Adenoviral Gene Delivery vs Lentiviral Gene Delivery

Use sucrase (sacB) to replace ccdB gene (Gateway cassette)

Comparison of Viral Delivery Systems

Plasmid map creation software

Delaware Company

pre-made Lentivirus

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loxP mutants

Taq DNA Polymerase

KAPA2G Fast PCR Kits – Faster than Usain Bolt

Lentivirus Production using GeneExpresso Max Transfection Reagent

Penn Vector Core AAV services

UPenn Vector Core AAV Services

Adeno-associated virus (AAV) Serotypes & applications

novapep.com Adenovirus and AAV Services

Sanford Burnham Medical Research Institut Lentivirus and Retrovirus Services

UTHSC VIRAL VECTOR CORE LABORATORY Price List

Adenovirus vectors from M.D. Anderson Cancer Center

Cyagen adenovirus services

Angio-Proteomie Custom Andenoviral Services

VIRAPUR Adenovirus Purification Kits

VIRAPUR Adenovirus and AAV Services

Puresyn Adenoserve® Contract Adenovirus Purification Service

norgenbiotek.com Custom Adenoviral Services

nitanbiotech.com Adenovirus, AAV and Lentivirus Services

Paragon Bioservices Adenovirus, AAV and Lentivirus Services

Eton Bioscience Adenovirus Products

mds-usa.com Virus Production & Purification Services

SIRION BIOTECH adenovirus and AAV products and services

ReGenX Biosciences AAV Product and Services Pricing List

welgeninc.com adenovirus, AAV and retrovirus products and services

genedetect.com rAVE (adeno-associated virus, AAV) products and services

lentivirus and AAV containing a bidirectional promoter for transgene expression in stem cells

Use EGFP-LoxP-RFP for Cre recombinase protein transfection

NCI Protein Expression Laboratory Services

National Cancer Institute at Frederick Advanced Technology Program (ATP)

NCI Antibody and Protein Purification Unit

NCI’s Antibody Characterization Program

Use muscle-optimized AAV-based antibody expression vector to fight HIV virus

Generate monoclonal antibodies using EBV

Adaptation of Cell Cultures to a Serum-Free Medium (Cellgro Protocol)

Adaptation of Cells to a Serum-Free Medium (Invitrogen Protocol)

Adaptation of Hela Cells From Monolayer to Suspension Culture

Grow HeLa cells in suspension

Common mammalian expression promoters

Human Kinase collection and 19000 human ORFs as premade lentivirus and adenovirus

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Develop an Adenovirus–Epstein-Barr Virus Hybrid Vector for Long Term Protein Expression

Making Monoclonal antibodies using Epstein-Barr virus and B lymphocytes

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EMPLOYEE NON-COMPETE AGREEMENT (example)

NON-DISCLOSURE AND CONFIDENTIALITY AGREEMENT (example)

Protect web directory with PHP

Agree to Assign vs. Hereby Assign

How to convert mysql timestamp to readable date format

A Simple, Rapid, Sensitive, Multiplex Diagnostic Chip for HIV, HBV, HCV and Syphilis Detection

Applications of phiC31 integrase

Set up a web application with Apache, PHP and MySQL on Amazon EC2

Amino Alcohols from Simga

Polyamines from Sigma

Piperazines from Sigma

HTML Codes: Characters and symbols

Gene synthesis of mammalian codon-optimized FLP gene and ΦC31 gene

Gateway Cloning Terminology

Reprogramming of Embryonic Fibroblasts with Stem Cell Factors (c-Myc, Klf4, Oct4, Sox2) in Sendai Virus Vector

Use Luminex xMAP® Technology for multiplex detection of HIV, HBV, HCV and syphilis

Thermostable Moloney Murine Leukaemia Virus Reverse Transcriptase

Protein Cross-linking using DSP and DTSSP

Protein Crosslinkers from Soltec Ventures

Use silicon dioxide for DNA Purification

How to Make 37430 Andy No Virus Online in One Day Using an Advanced Production System

Expression of Both Light-Chain and Heavy Chain as a Single-Open Reading Frame

Auto-synchronize files among Ubuntu computers using Dropbox

Monoclonal antibodies against KOD DNA polymerase

Chitosan-graft-PEI for transfection

What is PfuUltra and PfuTurbo

High pH Buffer for DNA polymerase

Purification of proteins from inclusion bodies

Stratagene’s Quick Change Site-Directed Mutagenesis Protocol

Useful epitope tag sequences

Purification of Proteins from Inclusion Bodies

Protocol for Amplification and Purification of Adenovirus

Adenovirus production protocol from Cecile Chartier and Calvin Kuo (Stanford University)

Avi-Tag, AviD-tag, Strep-Tag, nano-tag, SBP-Tag

E. coli promoters for protein expression

Cell Permeable TAT-HA2 Fusion Peptide for protein transfection

6x GRGreen agarose gel loading buffer

Comparison of herpes simplex virus, adenovirus, adeno-associated virus, Retrovirus and Lentivirus for gene delivery

Human ORF Clones from ORFeome Collaboration

Excellgen’s LvExpress Lentivirus Production and Purification

Excellgen’s AdExpress Adenovirus Production, Amplification and Purification

Use adenovirus for large scale production of adenovirus and lentivirus

Use adenovirus for large scale production of AAV with different serotypes

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Genome Engineering (gene editing) Methods

Use Adeno-Associated Virus (AAV) For Gene Editing

High level protein expression using Adenovirus-alphavirus hybrid virus

Asymmetric overlap extension PCR for mutagenesis or fusion of two PCR fragments

Fusion of three PCR fragments using Phusion or Deep Vent DNA Polymerases

Overlap extension PCR cloning of insert without restriction digestion

PCR terminology

Join two PCR fragements by T4 DNA ligase and Pfu DNA polymerase

Protein (antigenic) targets for developing of therapeutic human monoclonal antibodies

Bacterial Strains for Protein Expression

High processivity DNA Polymerases: Phusion, Herculase II Fusion, KAPAHiFi

Sulfolobus sp. DNA-binding protein (sac7d) fusion proteins

Pfu DNA Polymerase tricks

DNA Polymerase Fusion to increase enzyme processivity

KOD Dash DNA Polymerase

KOD DNA Polymerase

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Cancer Markers

How to remove chloramphenicol resistance gene from gateway cassette

Gene synthesis: codon otimization using Invitrogen’s GeneArt optimizer

High Fidelity Pfu DNA polymerase

Purification of Pfu DNA polymerase

Target site sequences of Cre, FLP and PhiC32 Recombinases

FLP and Cre recombinase

Endotoxin-free plasmid Gigaprep using Phamacia (GE) DEAE silica gel column

helper-dependent adenovirus production using FLPe recombinase

Adenovirus Vector maps: AdMax, Adeno-X, AdEasy, ViraPower, RAPAd

Qbiogene AdenoVator Adenovirus Production System Kits

Gene synthesis of mouse codon-optimized FLP gene (Flpo)

Gene synthesis of mammalian codon-optimized Cre recombinase

Virovek’s Adeno-associated virus (AAV) production Price list

de novo gene synthesis of adeno-associated virus capsid protein 9 (AAV9)

Adeno-associated Virus (AAV) serotype sequences

Inducible site-directed recombination using MerCreMer

Expression and Purification of Recombinant Tat-FLP Fusion Proteins

FLP-FRT recombination using Flippase recombination enzyme

Construction of helper adenovirus and use it to make helper-dependent adenovirus

Production of RCA-free adenovirus using Per.C6-E1 cells and Sigma medium

Measuring total particles and infectious units of purified helper-dependent adenovirus by qPCR

Measuring the physical titer of final HC-AdV preparations and helper virus contamination levels by qPCR

Measuring the physical titer of final HC-AdV preparations by optical density

Characterization and titration of helper-dependent adenovirus

Preamplification of helper-dependent adenovirus using adherent 116 cells

Amplification of helper-dependent adenovirus in suspension cultured 116 cells using a spinner flask culture system

Purification of high capacity helper-dependent adenovirus by ultracentrifugation

Production of helper-dependent adenovirus using GeneExpressoMax or Calcium phosphate transfection

Protocol for amplification and titering of helper adenovirus pAdHelper

Helper-dependent (high-capacity, gut-less) adenovirus features

Production of Helper-Dependent Adenovirus using GeneExpressoMax

Rapid one-step LR recombinational cloning using an enzyme mixture containing Int, IHF and Xis (Gateway Clonase) and two step PCR amplification

Gene Therapy Resources

National Gene Vector Biorepository (NGVB) AAV vector list

Effectiveness of signal peptides on protein expression

St. Jude Vector Laboratory Services

The Harvard Gene Therapy Initiative Viral Service Pricing

Sanford Burnham Medical Research Institute Viral Vector Services

University of Southern California CHLA vector Core Services

Johns Hopkins University Gene Therapy Vector Core Services

Quality Control of AAV, Adenovirus and Lentivirus

Baylor College of Medicine CAGT Vector Development Laboratory Pricing

Baylor College of Medicine CAGT Vector Development Laboratory SOP

Baylor College of Medicine CAGT Vector Development Laboratory Services

Univeristy of Florida Vector Core Laboratory AAV Service Pricing

University of the Connecticut Health Center Viral Vector Core Services

University of Pittsburgh Vector Core Retrovirus Services and Pricing

University of Pittsburgh Vector Core Adenoviruse Services and Pricing

Salk viral vector core products and services

University of Michigan Vector Core Retroviral Products and Services

University of Michigan Vector Core Lentivirus Products and Services

University of Michigan Vector Core Adenovirus Products and Services

QBiogene Adenovator adenovirus system features

Adenovirus Amplification and Purification using AdenoPure membrane

Adenovirus Purification by Ultracentrifugation (CsCl Gradient)

Premade Adenovirus from University of Iowa Gene Transfer Vector Core

UNC Vector Core Virus Services

Quality Control of Excellgen Adenoviral Vector Systems

Excellgen AdExpress™ Ready-to-use Recombinant Adenoviruses

Construction and Characterization of First-Generation Adenovirus Vectors (AdEasy Protocol)

Construction and Characterization of First-Generation Adenovirus Vectors (AdEasy Protocol)

Microbix Biosystems Adenovirus Kits Price List

Advantages of AdMax™ Adenoviral Vector Creation System (Microbix Biosystems)

O.D.260′s AdenoQuick™ Cloning System

QBiogene’s AdenoVator™ Adenoviral Vector System

Features of Qbiogene’s Adenovirus transfer vectors

QBioGene adenovirus AdenoVator system FAQ

QBioGene AdenoVator Adenoviral Vector System

AdMax Protocol: Adenoviral Vectors and 293 Cells FAQ

AdMax Protocol: Production and Purification of High Titer Adenovirus Stock

AdMax Protocol: Preparation of high-titer adenovirus crude virus stock

AdMax Adenovirus Protocol: plaque screening

AdMax Adenovirus Transfection Protocol

ADCre from Microbix Biosystems: Control of gene expression from Adenovirus vectors

AdMax HiIQ Adenovirus system from Microbix Biosystems for high level protein expression

Oncolytic Adenoviruses

AdMax™System from Microbix Biosystems for Production of Recombinant Adenovirus

Helper dependent (HD) (gutless, high capacity) adenovirus system from Microbix Biosystems

Invitrogen ViraPower™ Adenoviral Expression System Protocol

Clontech Adenovirus Purification Protocol

DNA Extraction with Phenol:Chloroform:Isoamyl Alcohol

Clontech Adenovirus Expression System 1 Adeno-X Protocol

Plaque Assay for Titering Adenovirus (AdEasy Protocol)

Adenovirus Production Protocol for AdEasy system

Adenovirus purification by ultracentrifugation

Generation of Adenovirus Pre-stocks

Properties of specific proteases for fusion protein cleavage

Plasmid DNA Purification using CTAB and Q Sepharose FF Anion Exchange column

Adenovirus QC

Titering Adenovirus using Clontech Adeno-X Rapid Titer Kit

Production of Retrovirus using GeneExpressoMax Transfection Reagent

State-of-the-art of hybrid vector technologies for viral gene therapy

Remove DNA from Crude Taq DNA Polymerase

Transfect suspension culture CHO and HEK293E cells with PEI

Transfect Suspension-adapted HEK293E cells with 25K PEI

Commonly used promoters for recombinant expression in E. coli

Cumate Operon

HiYield Bacterial medium

Adenovirus Plaque assay

Homemade recipe for 1L of Trizol

Introduction to Adenovirus Technology

High-titer Lentivirus Production for in vivo neural labeling

Lentiviral concentration by anion exchange chromatography using Mustang Q anion exchange membrane cartridges

Lentiviral concentration by anion-exchange chromatography and ultracentrifugation

Lentiviral concentration by ultracentrifugation

Lentivirus Purification by Ultracentrifuge

Concentrate Lentivirus by ultracentrifugation.

Lentivirus Production and Purification

Short 22nt IRES

Home-made Gateway Clonase II

Serum-Free Freezing medium for mammalian cells

Retrovirus Production using GeneExpressoMax

Regulated expression in mammalian cells

My protein production protocols (less than 10 easy steps)

Blunt end ligation protocol

MaxiPrep Plasmid Protocol using DMAE Anion-exchange Column

Lentivirus Production using 293SF-PacLV cells

Make a stable 293 cell line expressing repressor LacI

Amplification of adenovirus using 293F at low MOI

TAL Effector Publications

Purification of Plasmid DNA using CTAB on glass fiber spin column

Lentiviral infection Protocol

Amplification of adenovirus

Restriction Enzyme Compatible Cohesive Ends

NEBuffers for Double Restriction Enzyme Digestion

AAV Production and Purification Protocol

Purification of recombinant adenovirus by ultracentrifugation using CsCl gradient

Small-scale adenovirus amplification protocol

Plaque Assay for Purification and Titration of Recombinant Adenoviruses

Production of High Titer Adenoviral Stocks

Adenovirus Production using GeneExpresso Max Transfection Reagent

Antibiotic Selection Guide

Make mismatch-specific nuclease Cel-1 from celery

in vivo cleavage efficiency of 2A peptides

Gene Synthesis Error Removal using Cel-1 Nuclease from Celery

Recombinant adenovirus amplification protocol, Preparation of high titer adenovirus stocks

Taq Polymerase N-deletion mutants

Taq Polymerase N-deletion mutants

Genotypes of Invitrogen’s Competent Cells

001 The Development of Lateral Flow Immunochromatographic Devices to Detect Antibodies

Development of Induced Pluripotent Stem (iPS) Cell Lines for Use in Cell-based Bioassays

Generation and Qualification of Site-specific Post-translationally Modified Proteins for Use as Calibrators in Pharmacodynamic (PD) Assays

Development of Low Cost, Small Sample Multi-Analyte Technologies for Cancer Diagnosis, Prognosis and Early Detection

Difference between BL21 and BL21(DE3)

Purification of 6His TEV protease

VWR PCR Kits

Taq Polymerase PCR Enhancer

Qiagen’s LyseBlue solution

Gene synthesis, cloning and expression of a new bright and stable near-infrared flurescent protein

zinc-finger nuclease (ZFN) and transcription activator-like effectors (TALEN) for gene targeting

Improving DNA plasmid production in Escherichia coli

FPLC Purification of Plasmid DNA using Fractogel DEAE anion-exchange column

UPenn Vector Core Adeno-associated Virus and plasmid catalog

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BL21 cells for toxic protein expression

Purification of adeno-associated virus (AAV) by FPLC using heparin affinity column

Protocol for Adeno-associated virus (AAV) production using GeneExpresso Max

Center for Cancer Systems Biology (Dana Farber Cancer Institute)-Broad Human Kinase ORF collection

Structural Genomics Consortium Plasmids

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MultiSite Gateway Cloning Protocol

Purify PCR product using PEG 8000

Comparison of adenovirus, adeno-associated virus (AAV) and lentivirus

Relative in vitro Infectivity of AAV Vectors

Titration of Recombinant AAV by real-time PCR

Production of recombinant adeno-associated virus by CaCl2 Transfection: protocol 2

Purification of adeno-associated virus using Heparin Column

Production of recombinant adeno-associated virus by CaCl2 transfection

Purification of adeno-associated virus by heparin affinity chromatography

Purification of adeno-associated virus by iodixanol step gradient ultracentrifugation

Purify adeno-associated virus by CsCl ultracentrifugations

Large scale production of adeno-associated virus using GeneExpresso Max

Purification of Recombinant Rep Protein for Targeted Integration

Production of recombinant adeno-associated virus using GeneExpresso Max transfection reagent

Membrane anchored Gaussia luciferase for Bioluminescent cell imaging

Home made induced pluripotent stem cell using GeneExpresso Max and lentivirus

Isolating human neural stem cells from the human fetal brain

Use GeneExpresso Max to make 293 cell extract for autoantibody detection

Retrovirus production using GeneExpresso Max

Lentivirus Production using GeneExpresso Max

Commonly used Sequencing Primers

Human synapsin promoter for AAV, adenovirus and lentivirus vectors

Site-directed mutagenesis using Pfu DNA polymerase and DpnI

Subclone CDNA from plasmid to another plasmid without gel purificaiton

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Short synthetic internal ribosome entry site 22nt

Generate Stable Protein Expression Clones using Retrovirus

Protein Transfection using Pep-1 (Chariot, Active Motif)

5000 human proteins Protocol

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Retrovirus Production using GeneExpresso Max Transfection Reagent

Cre recombinase purification

Generate new AAC capsid genes by DNA shuffling

AAV Service Fees

Non-Integrating Lentiviral Vectors by Site-Directed Mutagenesis

Adeno-Associated vector production using GeneExpresso Max transfection reagent

Lentivirus Titering By Real Time PCR

Titration of Lentiviral Vectors by Reverse Transcriptase Activity

Properties of commonly used viral gene transfer vectors

Preparation of Calcium Competent Cells

Use of Excellgen Cre (NLS-Tat-Cre, HNC) recombinase for in vivo Lox-P recombination in cultured cells

Excellgen Protocol: FPLC Purification of Cre (Tat-NLS-Cre) recombinase for in vivo Lox-P recombination

FPLC purification of PKA alpha

In vitro Cre-Lox Recombination using Excellgen Cre Recombinase

Adenovirus and AAV from UPenn “Penn Vector Core”

Protocol for Adeno-associated Virus (AAV) Production using 293 cells

Purification of Plasmid DNA using Glass syringe filters

Protein Tools

Home-made Gel solubilization buffers

Oligo Design web sites for Gene Synthesis

Invitrogen PichiaPink Yeast Expression System advantages

Excellgen BioServices

Two-step Gene Synthesis (Two-Step Polymerase Construction Assembly)

Safety Protocols for Working in the Lentiviral Production Lab

Standard Operating Procedures (SOP) for Working Safely with Lentiviral Vectors

MMLV virus

Plasmid DNA purification using a Perfluorosorb S column

Low endotoxin plasmid Maxi-prep using cetyltrimethylammonium bromide and Tritonx-114

Diatomaceous earth plasmid maxiprep

Concentration of lentivirus by anion exchange chromatography using Mustang Q anion exchange membrane cartridges

Fast Plasmid DNA Isolation/Purification Methods

Homemade Buffers for plasmid kits

Home-made Miniprep kit

BIO International Convention 2011 Schedule

Sodium Phosphate Buffer Chart

Codon Usage in E. coli

Chemical modification of Taq Polymerase

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Autoinduce medium for bacterial protein expression

Conjugation of cholesterol to polyglycerol dendrimers

Taq polymerase purification Protocol

Protein Citraconylation Protocol

Reagents for protein re-folding

Regeneration of amylose-agarose beads

Sartobind Ion Exchange MA Protocol

Protein Production in Suspension Culture Hela Cells using Adenovirus

Amplification of adenovirus protocol: CsCl ultracentrifugation and FPLC purification

Protocol for titeration of adenovirus

High titer stock: Recombinant adenovirus amplification protocol

High titer recombinant adenovirus production

Caffeine can increase lentivirus production (up to 8 fold increase in lentivirus titer)

Pharmacia IEX Sepharose™ Fast Flow Technical Information

One step SYBR Green RT-PCR

Cell lysis buffer for RT-PCR

One Step RT-PCR Protocol

Use Cells-to-cDNA Cell Lysis Buffer for RT-PCR without RNA isolation

Homemade RNAzol Protocol for isolating RNA from culture cells.

Homemade TRIzol Protocol for isolating RNA from culture cells.

Increase protein stability and solubility using chaperones

Tips for purification of maltose-binding protein (MBP) fusion proteins

E Coli strains compatible with Invitrogen’s pBAD Expression Vector

Concentration of Lentiviral Supernatants by PEG Precipitation

Lentivirus purification using CIM DEAE monolithic column from BIA Separations

Moloney murine leukemia virus reverse transcriptase mutants

RT-qPCR method for lentivirus quantitation

Large-Scale Transient Transfection of CHO Cells for Protein Production

Infection of target cells with lentivirus

Enhence Calcium Phosphate mediated transfection using high molecular weight dextran

Glycerol shock for high efficiency calcium phosphate transfection

High Transfection Efficiency Protocol using Suspension Transfection Technique

Calcium Phosphate Transfection

Lentivirus Production using Calcium Phosphate Transfection

Lentivirus production using recombinant baculoviruses

Characteristics of commonly used gene transfer vectors

One-step real-time RT-qPCR for lentivirus quantitation

Lentiviral vector production using Cell Factories

Lentivirus production using polyethylemine (PEI)

Database for Chemicals

zinc/imidazole staining protocol for visualizing proteins in acrylamide gels

Protein Expression and Purification Protocols

Recombinant BoCPB: An Enzymatic Reagent for Removing Disordered, Positively Charged C-terminal Residues from Recombinant Proteins

A Universal Antigen Delivery Platform for Enhanced Immune Response

Purification of baculovirus with FPLC using Capto DEAE column or Sartobind D MA 75 membrane

Purify adenovirus by anion-exchange chromatography using FPLC

Purify lentivirus by anion-exchange chromatography-1

Purification of lentivirus using Millipore Fast-Trap Lentivirus Purification kit

Adenovirus Purification using Mustang Q membrane column

Sigma Protein Expression & Purification Services: list of prices

Titration of lentivirus using FACS

FPLC purification of lentivirus using anion-exchange membrane

High titer lentivirus production using GeneExpresso

Diafiltration for buffer exchange and desalting with ultrafiltration membrane using Millipore Pellicon tangential flow filtration cassettes

MTT assay of cellular toxicity (Cell Viability Assay)

Purification of lentivirus Using Mustang Q Ion Exchange

Custom high titer lentivirus production services

National Cancer Institute at Frederick (SAIC-Frederick) Protein Expression Laboratory PEL Price List

ELISA method for detecting autoantigen in human serum

MTT Assay of Cell Viability Protocol

Comparison of Real-time PCR (qPCR) Machines

Transfection of Adherent HEK293-EBNA1 Cells in a Six-Well Plate with Branched PEI for Production of Recombinant Proteins

Transfection of HEK293-EBNA1 Cells in Suspension with Linear PEI for Production of Recombinant Proteins

Sigma REDTaq DNA Polymerase

Conjugation of peptide to KLH

Cancer related genes

Multi-Analyte Profiling (MAP): biomarkers from Rules-Based Medicine

Cleaning and rehydration of Tosoh columns

Rhopalosiphum padi virus internal ribosome entry site

Tris-Tricine SDS-PAGE Buffer for RUN-Blue Gel

How to find out your Linksys Sipura’s IP address

Linksys Sipura SPA3102 SIP Configuration for Gafachi VOIP Service Using Static IP

Sipura SPA2000 SIP Configuration for Gafachi VOIP Service

Qiagen Endotoxin Free Plasmid Kit Buffer Composition

Home-made Qiagen Gel Extraction (Solubilization) QG Buffer

De Novo Gene Synthesis using Two-step PCR Cycling Assembly (PCA)

Use insect cells to make adeno-associated virus

Gene delivery using recombinant viruses: Baculovirus, Lentivirus, Herpesvirus, Adeno-Associated Virus,

shRNA design guidelines

Fluorescent Proteins from Evrogen

Lentivirus packaging using PEI

pGIPZ lentiviral shRNAmir protocol–Packaging Lentivirus

pGIPZ lentiviral shRNAmir Protocol–2

pGIPz lentiviral shRNAmir protocol

Patents for some DNA Polymerases

Custom adenovirus and adeno-associated virus (AAV) service providers

Self-cleaving 2A peptides & sequences

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LoxP Sequence for Cre Recombinase

plasmid copy number

Buffer for Qiagen Endotoxin Free Plasmid Kit

Patents for Fluorescent Proteins

Plasmid DNA Midiprep and Maxiprep using Glass Syringe Filter

Remove endotoxin from plasmid DNA using Triton X-114

Endofree Plasmid DNA purification using EMD Fractogel DMAE -650(M)

Plasmid DNA Binding Capacity of some anion exchange resins

Regerantion of Qiagen plasmid DNA purification columns using ascorbic acid and ferric chloride

Regeration of Qiagen miniprep, midiprep, maxiprep and megaprep columns

Self-cleaving 2A Peptides for gene fusion

Endo-free Qiagen plasmid miniprep and midiprep protocol

Remove endotoxin from plasmid DNA by anion exchange chromatography

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Regerantion of Qiagen plasmid DNA purification columns using ascorbic acid and ferric chloride

Regeration of Glutathione Sepharose Beads

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LIMS for Molecular Biologists or Small Biotech Companies

Miniprep plasmid DNA purification using diatomaceous earth or silica powder

Protocol for Preparing Chemical Competent Cells: Top10 & BL21

Rare codons in E coli BL21 (DE3)

Properties of red fluorescent proteins

How to design shRNA for pLKO.1-puro and TRC2-pLKO-puro

Gene synthesis by ligase chain reaction (LCR)

Oligo design software for gene synthesis

Sodium Borate, Lithium Borate and Lithium Acetate Buffers for Fast Agarose Gel Electrophoresis.

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Fluorescent Protein E2-Crimson

Western Blot Stripping Buffer & Protocol

Mutiple sites Mutagenesis using PCR

5 min rapid ligation protocol using 2X Quick Ligation Reaction Buffer

Fluorescent proteins

Buffer Compositions for Qiagen MiniPrep, Gel Purification and PCR Purification Kits

Sequences of some fluorescent proteins

Properties of New Fluorescent Proteins

Bright fluorescent proteins could be potentially used for transgenic organisms

Infrared-fluorescent proteins from Deinococcus radiodurans for whole-body imaging

SuperFolder YFP for in vitro Bimolecular Fluorescence Complementation Assay

How to design oligos for de novo gene synthesis of superfolder GFP

Comparison of new fluorescent proteins

Alignment of new red & far red fluorescent proteins

Comparison of Red & Far-Red Fluorescence Proteins

Soluble SuperFolder GFP

SuperFolder GFP

Gateway Cloning Primer Design Guide

Design Gateway Clone Primers using “Universal attB Primers” and “Universal attB Adapters”

How to Design Gateway attB Primers

Baculovirus Amplification Protocol

Infect Sf9 cells with baculovirus on plates

Bac-To-Bac Baculovirus expression systems Protocol

Phosphate buffer chart from Sigma

Phosphate buffer chart

Amplification of Baculoviral Stock

Amplification of Baculovirus Plaques and Preparation of High Titer Virus Stocks

How to Make High Titer Baculovirus Stocks

ultra-sensitive luciferase reporters

Buffer composition for Qiagen MiniPrep, midiPrep, MaxPrep, PCR clean up and gel extraction kits

Gateway Cloning Cassette Sequence

Restriction enzyme cleavage blocked by Dam and Dcm Methylation

Fluorescent Protein Properties

The Human Codon Usage Table

Serum Markers for Ovarian Cancer

Storage Buffer for Taq DNA Polymerase

Lentiviral vector: WPRE and cPPT

Cre recombination protocol

Commercial baculovirus expression vectors

Commercial bacterial expression vectors

Preferred stop codon for bacterial expression

Shine-Delgarno sequence

Promoters for expression of proteins in bacteria

The 8 least used codons in E. coli, yeast, Drosophila, and primates

MedImmune’s next generation proteins

Sequence of attB Expression Clone from Gateway LR reaction

Expression of unstable proteins in bacteria

Expression of Hydrophobic Proteins

Qiagen pQE vector for toxic protein

Qiagen pQE vector: Maintenance of the expression plasmid

PCR Protocol For Generating Optimized Templates for Thermo Human In vitro Expression Kits

PCR Protocol For Generating Optimized Templates for Thermo Human In vitro Expression Kits

Commonly used bacterial E.coli growth media

Screening of protein expression clone using Minimal Cell Culture Medium Glucose M9Y

Sticky PCR for protein engineering

Gene synthesis using overlapping oligos: PrimeSTAR HS high fidelity DNA polymerase from Takara is the winner

T7 endonuclease I treatment of PCR gene synthesis product

Gene Synthesis: Error removal using recombinant 6XHis-MBP-MutS from Thermus aquaticus

Gene synthesis: TopDown one-step gene assembly method

Buffer for gene synthesis error removal using Thermus thermophilus MutS protein

Gene Synthesis: Error removal by enzymatic cleavage of mismatches

Successful Gene Synthesis: Two-step overlap extension PCR

10 X Buffer for Long PCR using Taq DNA Polymerase

Gene Synthesis: Error correction using Thermus aquaticus Muts protein

Effective two step gene synthesis by PCR

Gene Synthesis: Protocol using Circular Assembly Amplification

Tris-Mops Fast SDS-PAGE Run Buffer 20x

Homo sapiens codon usage table

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Western Blot Using Nitro blue tetrazolium chloride and 5-Bromo-4-chloro-3-indolyl phosphate, NBT BCIP

Home-made Bradford reagent for protein assay

Home-Made Colloidal Silver Blue Protein Stain

Home-made sodium borate buffer for fast agarose gel electrophoresis

Home-made lithium borate buffer for fast agarose gel electrophoresis

Protein stain containing zincon (ZC) and ethyl violet

Sensitive Coomassie Brilliant Blue Stain containing aluminum sulfate

Home made Bis-Tris gel for protein electrophoresis, as good as NuPAGE gel

Purification (and refolding) of His-tagged recombinant protein from bacterial inclusion bodies

Cleaning nickle column with sodium hydroxide and hydrochloric acid

Cleaning Nickle Column with Guanidine HCl

Nickel Column Regeneration Protocol

FPLC buffers for Purifying Protein from Inclusion Body

In vitro loxP recombination reaction using Cre recombinase

Staining of Proteins in Gels with Coomassie Blue G-250 without Ethanol (or Methanol) and Acetic Acid

Clontech Creator DNA Cloning Procedure

Protocol for Making Calcium Chloride (CaCl2) Competent Cell

SDS_PAGE Analysis of Pilot Expression

Pilot Expression using pBAD expression system

Transform Top10 Chemical Competent Cells

Site-directed mutagenesis by PCR using Pfu DNA polymerase and DpnI

CloneEZ Recombination Protocol

Gateway Cloning BP and LR and Reactions

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